Modeling Cardiovascular Diseases with hiPSC-Derived Cardiomyocytes in 2D and 3D Cultures

被引:62
作者
Sacchetto, Claudia [1 ,2 ]
Vitiello, Libero [2 ,3 ]
de Windt, Leon J. [1 ]
Rampazzo, Alessandra [2 ]
Calore, Martina [1 ]
机构
[1] Univ Maastricht, Dept Mol Genet, Univ Singel 50, NL-6229 ER Maastricht, Netherlands
[2] Univ Padua, Dept Biol, Via Ugo Bassi 58B, I-35131 Padua, Italy
[3] Adm Headquarters Univ Perugia, Interuniv Inst Myol IIM, Piazza Lucio Severi 1, I-06132 Perugia, Italy
基金
欧洲研究理事会;
关键词
cardiac disease modeling; human induced pluripotent stem cells; 3D cardiac models; engineered heart tissue; PLURIPOTENT STEM-CELL; ENGINEERED HUMAN MYOCARDIUM; CARDIAC MICROTISSUES; QT SYNDROME; FUNCTIONAL MATURATION; METABOLIC MATURATION; ENDOTHELIAL-CELLS; IMPROVE RECOVERY; TISSUE; CARDIOMYOPATHY;
D O I
10.3390/ijms21093404
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the last decade, the generation of cardiac disease models based on human-induced pluripotent stem cells (hiPSCs) has become of common use, providing new opportunities to overcome the lack of appropriate cardiac models. Although much progress has been made toward the generation of hiPSC-derived cardiomyocytes (hiPS-CMs), several lines of evidence indicate that two-dimensional (2D) cell culturing presents significant limitations, including hiPS-CMs immaturity and the absence of interaction between different cell types and the extracellular matrix. More recently, new advances in bioengineering and co-culture systems have allowed the generation of three-dimensional (3D) constructs based on hiPSC-derived cells. Within these systems, biochemical and physical stimuli influence the maturation of hiPS-CMs, which can show structural and functional properties more similar to those present in adult cardiomyocytes. In this review, we describe the latest advances in 2D- and 3D-hiPSC technology for cardiac disease mechanisms investigation, drug development, and therapeutic studies.
引用
收藏
页数:32
相关论文
共 50 条
  • [41] Manipulation of 3D Cluster Size and Geometry by Release from 2D Micropatterns
    Leight, Jennifer L.
    Liu, Wendy F.
    Chaturvedi, Ritika R.
    Chen, Sophia
    Yang, Michael T.
    Raghavan, Srivatsan
    Chen, Christopher S.
    [J]. CELLULAR AND MOLECULAR BIOENGINEERING, 2012, 5 (03) : 299 - 306
  • [42] 2D and 3D Self-Assembling Nanofiber Hydrogels for Cardiomyocyte Culture
    Ikonen, Liisa
    Kerkela, Erja
    Metselaar, Gerald
    Stuart, Marc C. A.
    de Jong, Menno R.
    Aalto-Setala, Katriina
    [J]. BIOMED RESEARCH INTERNATIONAL, 2013, 2013
  • [43] Label-free cell segmentation of diverse tissues in 2D and 3D
    Wills, John W.
    Robertson, Jack
    Tourlomousis, Pani
    Gillis, Clare M. C.
    Barnes, Claire M.
    Miniter, Michelle
    Hewitt, Rachel E.
    Bryant, Clare E.
    Summers, Huw D.
    Powell, Jonathan J.
    Rees, Paul
    [J]. CELL REPORTS METHODS, 2023, 3 (02):
  • [44] Podosomes in space Macrophage migration and matrix degradation in 2D and 3D settings
    Wiesner, Christiane
    Le-Cabec, Veronique
    El Azzouzi, Karim
    Maridonneau-Parini, Isabelle
    Linder, Stefan
    [J]. CELL ADHESION & MIGRATION, 2014, 8 (03) : 179 - 191
  • [45] Comparative Analysis Between the 2D and 3D Models of Interstitial Microwave Hyperthermia
    Gas, Piotr
    Kurgan, Eugeniusz
    [J]. PROCEEDINGS OF 2016 17TH INTERNATIONAL CONFERENCE COMPUTATIONAL PROBLEMS OF ELECTRICAL ENGINEERING (CPEE), 2016,
  • [46] Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
    Feaster, Tromondae K.
    Casciola, Maura
    Narkar, Akshay
    Blinova, Ksenia
    [J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2022, (190):
  • [47] Gene expression data and FTIR spectra provide a similar phenotypic description of breast cancer cell lines in 2D and 3D cultures
    Smolina, Margarita
    Goormaghtigh, Erik
    [J]. ANALYST, 2018, 143 (11) : 2520 - 2530
  • [48] Comparative Transcriptome Analysis of Human Adipose-Derived Stem Cells Undergoing Osteogenesis in 2D and 3D Culture Conditions
    Kim, Byung-Chul
    Kwack, Kyu Hwan
    Chun, Jeewan
    Lee, Jae-Hyung
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (15)
  • [49] 3D bioprinting in the regenerative treatment of cardiovascular diseases Future or start of clinical translation?
    Berndt, Rouven
    [J]. ZEITSCHRIFT FUR HERZ THORAX UND GEFASSCHIRURGIE, 2021, 35 (06): : 364 - 369
  • [50] Mathematical modeling of cell growth in a 3D scaffold and validation of static and dynamic cultures
    Mokhtari-Jafari, Fatemeh
    Amoabediny, Ghassem
    Haghighipour, Nooshin
    Zarghami, Reza
    Saatchi, Alireza
    Akbari, Javad
    Salehi-Nik, Nasim
    [J]. ENGINEERING IN LIFE SCIENCES, 2016, 16 (03): : 290 - 298