Cellular Reprogramming: A New Technology Frontier in Pharmaceutical Research

被引：10

作者：

Brock, Amy ^{[2
]}

Goh, Hui-Tong ^{[1
]}

Yang, Binxia ^{[1
]}

Lu, Yu ^{[3
]}

Li, Hu ^{[2
,4
,5
,7
]}

Loh, Yuin-Han ^{[1
,6
]}

机构：

[1] A STAR Inst Med Biol, Epigenet & Cell Fates Lab, Singapore 138648, Singapore

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[4] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[5] Boston Univ, Ctr BioDynam, Boston, MA 02215 USA

[6] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[7] Harvard Univ, Sch Med, Wyss Inst, Boston, MA 02115 USA

来源：

PHARMACEUTICAL RESEARCH | 2012年 / 29卷 / 01期

关键词：

cellular reprogramming; drug discovery; iPSC; pharmaceutical research; stem cells; PLURIPOTENT STEM-CELLS; PREIMPLANTATION GENETIC DIAGNOSIS; ADULT HUMAN FIBROBLASTS; HUMAN SOMATIC-CELLS; HUMAN IPS CELLS; FUNCTIONAL CARDIOMYOCYTES; CRITICAL REGION; MOTOR-NEURONS; ANIMAL-MODELS; LONG-QT;

D O I：

10.1007/s11095-011-0618-z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Induced pluripotent stem cells via cellular reprogramming are now finding multiple applications in the pharmaceutical research and drug development pipeline. In the pre-clinical stages, they serve as model systems for basic research on specific diseases and then as key experimental tools for testing and developing therapeutics. Here we examine the current state of cellular reprogramming technology, with a special emphasis on approaches that recapitulate previously intractable human diseases in vitro. We discuss the technical and operational challenges that must be tackled as reprogrammed cells become incorporated into routine pharmaceutical research and drug discovery.

引用

页码：35 / 52

页数：18

共 104 条

[1] Direct Reprogramming of Adult Human Fibroblasts to Functional Neurons under Defined Conditions [J].

Ambasudhan, Rajesh ;

Talantova, Maria ;

Coleman, Ronald ;

Yuan, Xu ;

Zhu, Saiyong ;

Lipton, Stuart A. ;

Ding, Sheng .

CELL STEM CELL, 2011, 9 (02) :113-118

[2] Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency [J].

Anokye-Danso, Frederick ;

Trivedi, Chinmay M. ;

Juhr, Denise ;

Gupta, Mudit ;

Cui, Zheng ;

Tian, Ying ;

Zhang, Yuzhen ;

Yang, Wenli ;

Gruber, Peter J. ;

Epstein, Jonathan A. ;

Morrisey, Edward E. .

CELL STEM CELL, 2011, 8 (04) :376-388

[3] Opportunities for Use of Human iPS Cells in Predictive Toxicology [J].

Anson, B. D. ;

Kolaja, K. L. ;

Kamp, T. J. .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 2011, 89 (05) :754-758

[4] A LIVING SYSTEM ON A CHIP [J].

Baker, Monya .

NATURE, 2011, 471 (7340) :661-665

[5] Neuregulin1/ErbB4 Signaling Induces Cardiomyocyte Proliferation and Repair of Heart Injury [J].

Bersell, Kevin ;

Arab, Shima ;

Haring, Bernhard ;

Kuehn, Bernhard .

CELL, 2009, 138 (02) :257-270

[6] The developmental genetics of congenital heart disease [J].

Bruneau, Benoit G. .

NATURE, 2008, 451 (7181) :943-948

[7] Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome [J].

Carvajal-Vergara, Xonia ;

Sevilla, Ana ;

D'Souza, Sunita L. ;

Ang, Yen-Sin ;

Schaniel, Christoph ;

Lee, Dung-Fang ;

Yang, Lei ;

Kaplan, Aaron D. ;

Adler, Eric D. ;

Rozov, Roye ;

Ge, YongChao ;

Cohen, Ninette ;

Edelmann, Lisa J. ;

Chang, Betty ;

Waghray, Avinash ;

Su, Jie ;

Pardo, Sherly ;

Lichtenbelt, Klaske D. ;

Tartaglia, Marco ;

Gelb, Bruce D. ;

Lemischka, Ihor R. .

NATURE, 2010, 465 (7299) :808-U12

[8] Induced pluripotent stem cell models of the genomic imprinting disorders Angelman and Prader-Willi syndromes [J].

Chamberlain, Stormy J. ;

Chen, Pin-Fang ;

Ng, Khong Y. ;

Bourgois-Rocha, Fany ;

Lemtiri-Chlieh, Fouad ;

Levine, Eric S. ;

Lalande, Marc .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (41) :17668-17673

[9] Inactivation of Fac in mice produces inducible chromosomal instability and reduced fertility reminiscent of Fanconi anaemia [J].

Chen, M ;

Tomkins, DJ ;

Auerbach, W ;

McKerlie, C ;

Youssoufian, H ;

Liu, L ;

Gan, O ;

Carreau, M ;

Auerbach, A ;

Groves, T ;

Guidos, CJ ;

Freedman, MH ;

Cross, J ;

Percy, DH ;

Dick, JE ;

Joyner, AL ;

Buchwald, M .

NATURE GENETICS, 1996, 12 (04) :448-451

[10] Peripheral regeneration [J].

Chen, Zu-Lin ;

Yu, Wei-Ming ;

Strickland, Sidney .

ANNUAL REVIEW OF NEUROSCIENCE, 2007, 30 :209-233

← 1 2 3 4 5 6 7 8 9 10 →