Inhibitory effect of melanoidins from glucose-asparagine on carboxypeptidases activity

This paper shows the effect of melanodins obtained from a glucose/asparagine system and also the effect of excess of substrate on the activity of the carboxypeptidase A (CPA) and carboxypeptidase B (CPB) enzymes. It was found that the substrate might have an inhibiting effect at high concentration. The presence of melanoidins has an inhibitory effect on both enzymes, with the value of the maximum reaction rate of the enzymatic reaction decreasing with the increase in melanoidins, so that for CPA, a value of nil was obtained for the above-mentioned maximum reaction rate for melanoidin concentrations of 0.042% (w/v), while the maximum reaction rate value for CPB is nil for a concentration of 0.077% (w/v).