Direct electrochemistry and electrocatalysis of myoglobin immobilized in hyaluronic acid and room temperature ionic liquids composite film

被引：51

作者：

Zhang, Ya ^{[1
,2
]}

Zheng, Jianbin ^{[1
]}

机构：

[1] NW Univ Xian, Inst Analyt Sci, Shaanxi Prov Key Lab Electroanalyt Chem, Xian 710069, Shaanxi, Peoples R China

[2] Yulin Coll, Dept Chem & Chem Engn, Yulin 719000, Shaanxi, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 09期

基金：

中国国家自然科学基金;

关键词：

myoglobin; room temperature ionic liquid; hyaluronic acid; electrochemistry; electrocatalysis;

D O I：

10.1016/j.elecom.2008.07.022

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A novel biocomposite film based on hyaluronic acid (HA) and hydrophilic room temperature ionic liquid 1-ethyl-3-methyl-imidazolium tetrafluoroborate ([EMIM][BF4]) was explored. Here, HA was used as a binder to form [EMIM][BF4]-HA composite film and help [EMIM][BF4] to attaching on glass carbon electrode (GCE) surface, while doping [EMIM][BF4] in HA can effectively reduce the electron transfer resistance of HA. The composite film can be readily used as an immobilization matrix to entrap myoglobin (Mb). A pair of well-defined and quasi-reversible redox peaks of Mb was obtained at the Mb-[EMIM][BF4]-HA composite film modified GCE (Mb-[EMIM][BF4]-HA/GCE) through direct electron transfer between Mb and the underlying electrode. The Mb-[EMIM][BF4]-HA/GCE showed an excellent electrocatalytic activity toward the reduction of H2O2. Based on the [EMIM][BF4]-HA biocomposite film, a third-generation reagentless biosensor could be constructed for the determination of H2O2. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1400 / 1403

页数：4

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