Characterization of Echinostoma revolutum and Echinostoma robustum from ducks in Bangladesh based on morphology, nuclear ribosomal ITS2 and mitochondrial nad1 sequences

被引:18
作者
Mohanta, Uday Kumar [1 ,2 ]
Watanabe, Takuya [1 ]
Anisuzzaman [3 ]
Ohari, Yuma [1 ,2 ]
Itagaki, Tadashi [1 ,2 ]
机构
[1] Iwate Univ, Lab Vet Parasitol, Fac Agr, Ueda 3-18-8, Morioka, Iwate 0208550, Japan
[2] Gifu Univ, Dept Pathogen Vet Sci, United Grad Sch Vet Sci, Yanagido 1-1, Gifu 5011193, Japan
[3] Bangladesh Agr Univ, Dept Parasitol, Mymensingh 2202, Bangladesh
关键词
Echinostoma revolutum; Echinostoma robustum; ITS2; nad1; Bangladesh; PHYLOGENETIC-RELATIONSHIPS; MIYAGAWAI ISHII; LIFE-CYCLE; TREMATODA; DIGENEA; DNA; REDESCRIPTION; INFERENCE; PARASITES;
D O I
10.1016/j.parint.2018.11.002
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Precise discrimination of Echinostoma species within the 'revolutum' group is quite difficult because of their morphological similarities. The objective of this study was to precisely characterize the echinostomes of ducks from Bangladesh based on both morphological and molecular characteristics. Two Echinostoma species were identified: E. revolutum and E. robustum. In the phylogenetic trees (ITS2 and nad1), E. revolutum and E. robustum belonged to their respective Eurasian Glade, which is distinct from the American Glade. These results suggest that both species have two distinct and geographically separated lineages, Eurasian and American. Our molecular and morphological data combined with previously published data supports the synonymy of E. robustum, E. miyagawai, and E. friedi previously based on either molecular or morphological evidence. This study thus improves our understanding of species diversity of the 'revolutum' group, particularly in Asia.
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页码:1 / 7
页数:7
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