The classification criteria are established to classify the water of Taihu Lake into four classes based on above-water remote sensing reflectance (R-rs), i.e., types A to D. Among the four water types, type A spectra represented the case of waters where algal blooms or aquatic plants appeared, while type B is referred to the water with high suspended matter concentration and low chlorophyll a concentration (C-chla). Both types A and B were not suitable for retrieving C-chla from image data. Hence, three-band, four-band, and two-band ratio algorithms were constructed to retrieve Cchla from water types C and D. The obtained results showed that the relation trends between C-chla and R-rs were different between type C and type D waters. By using Medium Resolution Imaging Spectrometer images, acquired on November 11, 2007 and November 20, 2008, the C-chla of Taihu Lake was mapped by band 9/band 7 models; it could be concluded that the C-chla mainly ranged from 0 to 20 mg . m(-3), accounting for 83.70% of the whole lake area in 2007 image, while the area was 86.63% in 2008 image. The estimation accuracies varied from different C-chla ranges. The mean absolute percent errors obtained by band 9/band 7 models were 106.23%, 56.79%, 38.04%, 33.80%, and 58.74% for the ranges 0 mg . m(-3) < C-chla < = 5 mg . m(-3), 5 mg . m(-3) < C-chla < = 10 mg . m(-3), 10 mg . m(-3) < C-chla < = 20 mg . m(-3), 20 mg . m(-3) < C-chla < = 30 mg . m(-3), and 30 mg . m(-3) < C-chla, respectively. Correspondingly, the root-mean-square errors were 5.02, 4.45, 5.59, 8.72, and 32.55 mg . m(-3), respectively.