Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season

被引：72
作者：
Shrivastava, Shubham [1 ]
Tiraki, Divya [1 ]
Diwan, Arundhati [2 ]
Lalwani, Sanjay K. [3 ]
Modak, Meera [4 ]
Mishra, Akhilesh Chandra [1 ]
Arankalle, Vidya A. [1 ]
机构：
[1] Bharati Vidyapeeth Deemed Univ, Interact Res Sch Hlth Affairs, Dept Communicable Dis, Pune, Maharashtra, India
[2] Bharati Vidyapeeth Deemed Univ Med Coll, Dept Med, Pune, Maharashtra, India
[3] Bharati Vidyapeeth Deemed Univ Med Coll, Dept Pediat, Pune, Maharashtra, India
[4] Bharati Vidyapeeth Deemed Univ Med Coll, Dept Microbiol, Pune, Maharashtra, India
来源：
PLOS ONE | 2018年 / 13卷 / 02期
关键词：
RIO-DE-JANEIRO; HEMORRHAGIC-FEVER; EMERGENCE; TYPE-3; DELHI; EVOLUTION; OUTBREAK; PHYLOGEOGRAPHY; REEMERGENCE; TRAVELERS;
D O I：
10.1371/journal.pone.0192672
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Dengue is the most common mosquito-borne viral infection in tropical and sub-tropical countries. In recent years, India has reported increased incidences of concurrent infection with multiple serotypes of dengue viruses (DENV). In the present study, we have characterized DENV circulating during a single season of 2016 in Pune, India. A total of 64 serum samples from NS1 ELISA positive dengue patients were used for PCR amplification of CprM region of the viral genome and sequencing. Phylogenetic analysis documented circulation of all the four DENV serotypes with predominance of DENV-2 (40.6%). DENV genotyping classified DENV-1 to Genotype V, DENV-2 to Genotype IV, DENV-3 to Genotype III and DENV-4 to Genotype I. Further analysis revealed emergence of a novel clade (D) of genotype I of DENV-4. Subsequent isolation of three DENV-4 viruses in cell culture followed by complete genome sequence analysis confirmed this observation. Additionally, a new genotype within serotype-4 with >6.7% sequence variation from other genotypes was identified. This first report of significant co-circulation of all the four serotypes in a single outbreak in Pune reconfirms need for molecular monitoring of DENV.
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页数：19
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