Kinetic Schemes for Post-Synchronized Single Molecule Dynamics

被引：20

作者：

Chen, Chunlai

Greenberg, Michael J.

Laakso, Joseph M.

Ostap, E. Michael

Goldman, Yale E. ^{[1
]}

Shuman, Henry

机构：

[1] Univ Penn, Penn Muscle Inst, Perelman Sch Med, Philadelphia, PA 19104 USA

来源：

BIOPHYSICAL JOURNAL | 2012年 / 102卷 / 06期

基金：

美国国家卫生研究院;

关键词：

TRANSFER-RNA; MYOSIN-I; TRANSLOCATION; RIBOSOME; MOTOR;

D O I：

10.1016/j.bpj.2012.01.054

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics leading up to a synchronized event, determined by the lifetimes of the preceding states; however, are not as obvious if the reaction has reversible steps or branches. Here we describe a general procedure for dealing with these situations.

引用

收藏

页码：L23 / L25

页数：3

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