Linking Specific Heterotrophic Bacterial Populations to Bioreduction of Uranium and Nitrate in Contaminated Subsurface Sediments by Using Stable Isotope Probing

被引:11
作者
Akob, Denise M. [1 ]
Kerkhof, Lee [2 ]
Kuesel, Kirsten [3 ]
Watson, David B. [4 ]
Palumbo, Anthony V. [4 ]
Kostka, Joel E. [1 ]
机构
[1] Florida State Univ, Tallahassee, FL 32306 USA
[2] Rutgers State Univ, Inst Marine & Coastal Sci, New Brunswick, NJ 08901 USA
[3] Univ Jena, Inst Ecol, D-07743 Jena, Germany
[4] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA
关键词
SCALE PHYSICAL MODELS; MICROBIAL COMMUNITIES; U(VI) REDUCTION; DENITRIFYING BACTERIA; REDUCING CONDITIONS; U(IV) OXIDATION; AQUIFER; BIOREMEDIATION; BIOSTIMULATION; DIVERSITY;
D O I
10.1128/AEM.05247-11
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [(13)C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.
引用
收藏
页码:8197 / 8200
页数:4
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