Discovery and characterization of circulating tumor cell clusters in neuroendocrine tumor patients using nanosubstrate-embedded microchips

被引：12

作者：

Sun, Na ^{[1
,2
]}

Yang, Yingying ^{[3
,4
]}

Miao, Hui ^{[3
,4
]}

Redublo, Peter ^{[3
]}

Liu, Hongtao ^{[1
]}

Liu, Wenfei ^{[5
]}

Huang, Yen-Wen ^{[1
]}

Teng, Pai-Chi ^{[6
]}

Zhang, Ceng ^{[1
,7
]}

Zhang, Ryan Y. ^{[1
]}

Smalley, Matthew ^{[1
]}

Yang, Peng ^{[1
]}

Chou, Shih-Jie ^{[1
]}

Huai, Kevin ^{[1
]}

Zhang, Zhicheng ^{[1
]}

Lee, Yi-Te ^{[1
]}

Wang, Jasmine J. ^{[6
]}

Wang, Jing ^{[1
]}

Liang, Icy Y. ^{[1
]}

Zhang, Tiffany X. ^{[1
]}

Zhang, Dongyun ^{[3
]}

Liang, Li

Weiss, Paul S. ^{[5
]}

Posadas, Edwin M. ^{[6
]}

Donahue, Timothy ^{[8
]}

Hecht, J. Randolph ^{[9
]}

Allen-Auerbach, Martin S. ^{[10
]}

Bergsland, Emily K. ^{[11
]}

Hope, Thomas A. ^{[12
]}

Pei, Renjun ^{[2
]}

Zhu, Yazhen ^{[1
]}

Tseng, Hsian-Rong ^{[1
]}

Heaney, Anthony P. ^{[3
]}

机构：

[1] UCLA, David Geffen Sch Med, Dept Mol & Med Pharmacol, Crump Inst Mol Imaging,Calif NanoSyst Inst, Los Angeles, CA 90095 USA

[2] Univ Chinese Acad Sci, Chinese Acad Sci, Suzhou Inst NanoTech & Nanobinon, Key Lab Nanobio Interface, Suzhou 215123, Peoples R China

[3] UCLA, Dept Med, Div Endocrinol Diabet & Hypertens, David Geffen Sch Med, Los Angeles, CA 90095 USA

[4] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Key Lab Endocrinol Natl Hlth Commiss, Dept Endocrinol, Beijing 100730, Peoples R China

[5] Univ Calif Los Angeles, Calif NanoSyst Inst, Dept Chem & Biochem, Dept Mat Sci & Engn,Dept Bioengn, Los Angeles, CA 90095 USA

[6] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Los Angeles, CA 90048 USA

[7] Southern Med Univ, Dept Pathol, Guangzhou 510515, Guangdong, Peoples R China

[8] UCLA, Dept Surg, David Geffen Sch Med, Los Angeles, CA 90095 USA

[9] UCLA, Dept Med, Div Hematol Oncol, David Geffen Sch Med, Los Angeles, CA 90095 USA

[10] UCLA, Dept Mol & Med Pharmacol, David Geffen Sch Med, Los Angeles, CA 90095 USA

[11] Univ Calif San Francisco, Helen Diller Family Comprehens Canc Ctr, Dept Clin Med, San Francisco, CA 94158 USA

[12] Univ Calif San Francisco, Dept Radiol & Biomed Imaging, San Francisco, CA 94158 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2022年 / 199卷

基金：

美国国家卫生研究院;

关键词：

Circulating tumor cells; Circulating tumor cell clusters; Nanostructured substrates; Neuroendocrine tumor; Peptide receptor radionuclide therapy; RECEPTOR RADIONUCLIDE THERAPY; EFFICIENT CAPTURE; BREAST-CANCER; DISEASE; ARRAYS; ASSAY;

D O I：

10.1016/j.bios.2021.113854

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Circulating tumor cell (CTC) clusters are present in cancer patients with severe metastasis, resulting in poor clinical outcomes. However, CTC clusters have not been studied as extensively as single CTCs, and the clinical utility of CTC clusters remains largely unknown. In this study, we aim sought to explore the feasibility of NanoVelcro Chips to simultaneously detect both single CTCs and CTC clusters with negligible perturbation to their intrinsic properties in neuroendocrine tumors (NETs). We discovered frequent CTC clusters in patients with advanced NETs and examined their potential roles, together with single NET CTCs, as novel biomarkers of patient response following peptide receptor radionuclide therapy (PRRT). We observed dynamic changes in both total NET CTCs and NET CTC cluster counts in NET patients undergoing PRRT which correlated with clinical outcome. These preliminary findings suggest that CTC clusters, along with single CTCs, offer a potential noninvasive option to monitor the treatment response in NET patients undergoing PRRT.

引用

页数：9

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