CircVRK1 regulates tumor progression and radioresistance in esophageal squamous cell carcinoma by regulating miR-624-3p/PTEN/PI3K/AKT signaling pathway

被引：68

作者：

He, Yunlong ^{[1
]}

Mingyan, E. ^{[1
]}

Wang, Chunbo ^{[1
]}

Liu, Guohui ^{[1
]}

Shi, Manru ^{[1
]}

Liu, Shuang ^{[2
]}

机构：

[1] Harbin Med Univ, Canc Hosp, Dept Radiat Oncol, 150 Haping Rd, Harbin 150060, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Dept Gynecol, Canc Hosp, 150 Haping Rd, Harbin 150060, Heilongjiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 125卷

关键词：

circVRK1; ESCC; PTEN/PI3K/AKT pathway; COMPETING ENDOGENOUS RNA; PROGNOSTIC MARKER; PTEN;

D O I：

10.1016/j.ijbiomac.2018.11.273

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a novel class of noncoding RNAs (ncRNAs), circular RNAs (circRNAs) have been verified to be potential biomarkers and therapeutic targets for human malignant tumors. However, the thorough understanding of circRNAs in the progression of esophageal squamous cell carcinoma (ESCC) still needs to be improved. This study focused on exploring the function and mechanism of circVRK1 in ESCC. At first, we examined the expression level of circVRK1 in ESCC tissues and cell lines with qRT-PCR. We found that circVRK1 was downregulated in ESCC tissues and cell lines. Kaplan-Meier method was used to analyze the correlation between circVRK1 expression and the overall survival of ESCC patients. Functionally, overexpression of circVRK1 suppressed the cell proliferation, migration and epithelial-mesenchymal transition (EMT) and reversed the radioresistance. Therefore, we identified the tumor suppressive role of circVRK1 in ESCC progression. Mechanistically, circVRK1 positively regulated PTEN by acting as a molecular sponge of miR-624-3p. Moreover, circVRK1 decreased the activity of PI3K/AKT signaling pathway by upregulating PTEN. Rescue assays were carried out to confirm the function of circVRK1-miR-624-3p-PTEN axis in ESCC progression. Our findings showed that circVRK1 suppressed ESCC progression by regulating miR-624-3p/PTEN axis and PI3K/AKT signaling pathway, suggesting the potential therapeutic value of circVRK1 for ESCC. (C)2018 Elsevier B.V. All rights reserved.

引用

页码：116 / 123

页数：8

共 31 条

[1]

[Anonymous], 2018, ANTI-CANCER DRUG, DOI [DOI 10.3322/caac.20115, DOI 10.1097/CAD.0000000000000617]

[2] circEPSTI1 as a Prognostic Marker and Mediator of Triple-Negative Breast Cancer Progression [J].

Chen, Bo ;

Wei, Weidong ;

Huang, Xiaojia ;

Xie, Xinhua ;

Kong, Yanan ;

Dai, Danian ;

Yang, Lu ;

Wang, Jin ;

Tang, Hailin ;

Xie, Xiaoming .

THERANOSTICS, 2018, 8 (14) :4003-4015

[3] Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer [J].

Chen, Jie ;

Li, Yan ;

Zheng, Qiupeng ;

Bao, Chunyang ;

He, Jian ;

Chen, Bin ;

Lyu, Dongbin ;

Zheng, Biqiang ;

Xu, Yu ;

Long, Ziwen ;

Zhou, Ye ;

Zhu, Huiyan ;

Wang, Yanong ;

He, Xianghuo ;

Shi, Yingqiang ;

Huang, Shenglin .

CANCER LETTERS, 2017, 388 :208-219

[4] Medical progress - Esophageal cancer [J].

Enzinger, PC ;

Mayer, RJ .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 349 (23) :2241-2252

[5] PTEN/PTENP1: 'Regulating the regulator of RTK-dependent PI3K/Akt signalling', new targets for cancer therapy [J].

Haddadi, Nahal ;

Lin, Yiguang ;

Travis, Glena ;

Simpson, Ann M. ;

McGowan, Eileen M. ;

Nassif, Najah T. .

MOLECULAR CANCER, 2018, 17

[6] Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis [J].

Hsiao, Kuei-Yang ;

Lin, Ya-Chi ;

Gupta, Sachin Kumar ;

Chang, Ning ;

Yen, Laising ;

Sun, H. Sunny ;

Tsai, Shaw-Jenq .

CANCER RESEARCH, 2017, 77 (09) :2339-2350

[7]

ISONO K, 1991, ONCOLOGY, V48, P411

[8] The role of HGF-MET pathway and CCDC66 cirRNA expression in EGFR resistance and epithelial-to-mesenchymal transition of lung adenocarcinoma cells [J].

Joseph, Nithila A. ;

Chiou, Shiow-Her ;

Lung, Zoe ;

Yang, Cheng-Lin ;

Lin, Tze-Yi ;

Chang, Hui-Wen ;

Sun, H. Sunny ;

Gupta, Sachin Kumar ;

Yen, Laising ;

Wang, Shulhn-Der ;

Chow, Kuan-Chih .

JOURNAL OF HEMATOLOGY & ONCOLOGY, 2018, 11

[9] MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells [J].

Matsushima, Kayoko ;

Isomoto, Hajime ;

Yamaguchi, Naoyuki ;

Inoue, Naoki ;

Machida, Haruhisa ;

Nakayama, Toshiyuki ;

Hayashi, Tomayoshi ;

Kunizaki, Masaki ;

Hidaka, Shigekazu ;

Nagayasu, Takeshi ;

Nakashima, Masahiro ;

Ujifuku, Kenta ;

Mitsutake, Norisato ;

Ohtsuru, Akira ;

Yamashita, Shunichi ;

Korpal, Manav ;

Kang, Yibin ;

Gregory, Philip A. ;

Goodall, Gregory J. ;

Kohno, Shigeru ;

Nakao, Kazuhiko .

JOURNAL OF TRANSLATIONAL MEDICINE, 2011, 9

[10] Esophageal cancers: Changing character, increasing incidence [J].

McCann, J .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1999, 91 (06) :497-498

← 1 2 3 4 →