Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales

被引：26

作者：

Arata, Paula X. ^{[1
]}

Quintana, Irene ^{[2
]}

Canelon, Dilsia J. ^{[3
]}

Vera, Beatriz E. ^{[4
]}

Compagnone, Reinaldo S. ^{[5
]}

Ciancia, Marina ^{[1
,6
]}

机构：

[1] Univ Buenos Aires, Fac Agron, Catedra Quim Biomol, Dept Biol Aplicada & Alimentos, RA-1053 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Lab Hemostasia & Trombosis, Dept Quim Biol, Fac Ciencias Exactas & Nat, RA-1053 Buenos Aires, DF, Argentina

[3] Cent Univ Venezuela, Fac Med, Escuela Bioanal, Caracas 1051, Venezuela

[4] Cent Univ Venezuela, Fac Ciencias, Escuela Quim, Caracas 1450, Venezuela

[5] Cent Univ Venezuela, Inst Expt Biol, Ctr Bot Trop, Lab Ecol & Taxon Macrofitas Marinas, Caracas, Venezuela

[6] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, CIHIDECAR CONICET, RA-1053 Buenos Aires, DF, Argentina

来源：

CARBOHYDRATE POLYMERS | 2015年 / 122卷

关键词：

Sulfated galactan; Pyruvic acid ketal; Green seaweed; Anticoagulant activity; Fibrin formation; CODIUM-YEZOENSE BRYOPSIDALES; ALGA CAULERPA-CUPRESSOIDES; CELL-WALLS; POLYSACCHARIDES; RACEMOSA; FRAGILE; CHLOROPHYTA; COMPONENTS; SEPARATION; HEPARIN;

D O I：

10.1016/j.carbpol.2014.10.030

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1'-carboxy)ethylidene-D-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from Penicillus capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：376 / 386

页数：11

共 52 条

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