Characterization of a TnMAVS protein from Tetraodon nigroviridis

被引：22

作者：

Xiang, Zhiming ^{[1
,2
]}

Qi, Lin ^{[1
]}

Chen, Weijian ^{[1
]}

Dong, Chuanfu ^{[1
]}

Liu, Zhaoyu ^{[1
]}

Liu, Dong ^{[1
]}

Huang, Mengli ^{[1
]}

Li, Wei ^{[1
]}

Yang, Gan ^{[1
]}

Weng, Shaoping ^{[1
]}

He, Jianguo ^{[1
]}

机构：

[1] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[2] Fuzhou Univ, Inst Life Sci, Fuzhou 350108, Fujian, Peoples R China

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2011年 / 35卷 / 11期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

TnMAVS; Interferon-sensitive response element; NF-kB; ISKNV; NF-KAPPA-B; DOUBLE-STRANDED-RNA; INNATE IMMUNE-RESPONSE; ANTIVIRAL SIGNALING PROTEIN; TOLL-LIKE RECEPTOR-3; ADAPTER PROTEIN; INTERFERON; INDUCTION; ACTIVATION; VIRUS;

D O I：

10.1016/j.dci.2011.03.029

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A growing family of cellular proteins encoding for caspase activation and the recruitment domain (CARD) plays a crucial role in immunity by sensing viral infections and signaling antiviral immune defenses. We obtained a MAVS-like protein (named TnMAVS) from Tetradon nigroviridis. which contains a CARD domain, a pro-rich domain, and a TM domain similar to human MAVS. A fluorescence assay showed that TnMAVS was located in the cytoplasm and near by the membrane, and not the mitochondria in FHM cells. As such, it was considered as a new member of MAVS. The TnMAVS was highly expressed in the liver and muscle of T. nigroviridis. In the spleen, TnMAVS was down-regulated when the fish was treated with polyinosinic:polycytidylic acid or challenged with ISKNV, but was not affected by PGN or LPS. The dual luciferase reporter assay revealed that TnMAVS overexpression resulted in the activation of the interferon-sensitive response element and NF-kappa B signal pathways. In addition, a characteristic TRAF3-associated peptide PVQD was found in the TnMAVS sequence. Co-immunoprecipitation assays indicated that TnMAVS could interact with zfTRAF3 in eukaryotic cells. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1103 / 1115

页数：13

共 23 条

[1] Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3 [J].