Two methods of temperature control for single-molecule measurements

被引：9
|
作者：
Baker, Matthew A. B. [1 ]
Inoue, Yuichi [2 ]
Takeda, Kuniaki [2 ]
Ishijima, Akihiko [2 ]
Berry, Richard M. [1 ]
机构：
[1] Univ Oxford, Clarendon Lab, Oxford OX1 3DP, England
[2] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Sendai, Miyagi 980, Japan
来源：
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2011年 / 40卷 / 05期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Temperature; Single molecule; Microscopy; Bacterial flagellar motor; Myosin; FLAGELLAR MOTOR; ESCHERICHIA-COLI; DEPENDENCE; ACTIVATION; MOVEMENT; ROTATION; ENERGY; SIZE;
D O I：
10.1007/s00249-010-0667-y
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.
引用
收藏
页码：651 / 660
页数：10
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