Directed mutagenesis confirms the functional importance of positively selected sites in polygalacturonase inhibitor protein

被引：38
作者：
Bishop, JG
机构：
[1] Max Planck Inst Chem Ecol, D-07745 Jena, Germany
[2] Washington Univ, Sch Biol Sci, St Louis, MO 63130 USA
来源：
MOLECULAR BIOLOGY AND EVOLUTION | 2005年 / 22卷 / 07期
关键词：
polygalacturonase inhibitor protein; directed mutagenesis; bean; Phaseolus vulgaris; positive selection; codon-substitution models;
D O I：
10.1093/molbev/msi146
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polygalacturonase inhibitor proteins (PGIPs) protect plants against invasion by diverse microbial and invertebrate enemies that use polygalacturonase (PG) to breach the plant cell wall. Directed mutagenesis has identified specific natural mutations conferring novel defensive capability in green bean PGIP against a specific fungal PG. These same sites are identified as positively selected by phylogenetic codon-substitution models, demonstrating the utility of such models for connecting retrospective comparative analyses with contemporary, ecologically relevant variation.
引用
收藏
页码：1531 / 1534
页数：4
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