Interfacing Cell-Based Assays in Environmental Scanning Electron Microscopy Using Dielectrophoresis

被引：23

作者：

Khoshmanesh, Khashayar ^{[1
,2
,3
]}

Akagi, Jin ^{[1
,2
]}

Nahavandi, Saeid ^{[3
]}

Kalantar-zadeh, Kourosh ^{[4
]}

Baratchi, Sara ^{[5
]}

Williams, David E. ^{[1
,2
]}

Cooper, Jonathan M. ^{[6
]}

Wlodkowic, Donald ^{[1
,2
]}

机构：

[1] Univ Auckland, Dept Chem, Auckland, New Zealand

[2] Univ Auckland, MacDiarmid Inst Adv Mat & Nanotechnol, Auckland 1, New Zealand

[3] Deakin Univ, Ctr Intelligent Syst Res, Waurn Ponds, Vic, Australia

[4] RMIT Univ, Sch Elect & Comp Engn, Melbourne, Vic, Australia

[5] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia

[6] Univ Glasgow, Dept Elect & Elect Engn, Bioelect Res Ctr, Glasgow G12 8QQ, Lanark, Scotland

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 08期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

CYTOMETRY;

D O I：

10.1021/ac2002142

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work, provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.

引用

页码：3217 / 3221

页数：5

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