Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis

被引：8

作者：

Allen, K. J. ^{[1
]}

Tennant, K. V. ^{[1
,3
]}

Franklin, S. H. ^{[2
]}

机构：

[1] Univ Bristol, Bristol Vet Sch, Langford House, Bristol BS40 5DU, Avon, England

[2] Univ Adelaide, Sch Anim & Vet Sci, Equine Hlth & Performance Ctr, Roseworthy, SA 5371, Australia

[3] Dick White Referrals, Stn Farm, London Rd, Six Mile Bottom CB8 0UH, Cambs, England

来源：

VETERINARY JOURNAL | 2019年 / 254卷

关键词：

Bronchoalveolar lavage; Cytology; Epithelial cell; Horse; Tracheal wash; INFLAMMATORY AIRWAY DISEASE; BRONCHOALVEOLAR LAVAGE; RACEHORSES;

D O I：

10.1016/j.tvjl.2019.105405

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Published studies vary as to whether epithelial cells are included in differential counts for tracheal wash (TW) and bronchoalveolar lavage (BAL) cytology in horses. The aim of this study was to determine whether inclusion or exclusion of epithelial cells affects interpretation of airway cytology. Using criteria of >20% TW neutrophils, >10% BAL neutrophils and/or >5% BAL mast cells to indicate airway inflammation, there was a change in categorisation from 'normal' to 'abnormal' in 21%, 4% and 8% horses, respectively, when epithelial cells were excluded from differential counts. It is recommended that future equine respiratory research studies explicitly state whether epithelial cells are included or excluded in differential counts. A consensus on epithelial cell inclusion during cytology reporting is required. (C) 2019 Elsevier Ltd. All rights reserved.

引用

页数：2

共 12 条

[1] A case-control study of respiratory disease in Thoroughbred racehorses in Sydney, Australia [J].

Christley, RM ;

Hodgson, DR ;

Rose, RJ ;

Wood, JLN ;

Reid, SWJ ;

Whitear, KG ;

Hodgson, JL .

EQUINE VETERINARY JOURNAL, 2001, 33 (03) :256-264

[2] Inflammatory Airway Disease of HorsesRevised Consensus Statement [J].

Couetil, L. L. ;

Cardwell, J. M. ;

Gerber, V. ;

Lavoie, J-P. ;

Leguillette, R. ;

Richard, E. A. .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 2016, 30 (02) :503-515

[3]

da Silva Katia Moreira, 2017, Journal of Equine Science, V28, P55, DOI 10.1294/jes.28.55

[4]

Davis MS, 2002, EQUINE VET J, V34, P413

[5] Reliability of 400-cell and 5-field leukocyte differential counts for equine bronchoalveolar lavage fluid [J].

Fernandez, Nicole J. ;

Hecker, Kent G. ;

Gilroy, Cornelia V. ;

Warren, Amy L. ;

Leguillette, Renaud .

VETERINARY CLINICAL PATHOLOGY, 2013, 42 (01) :92-98

[6]

Hodgson J.L., 2002, HAVEMEYER FDN MONOGR, V9, P49

[7] Comparison of tracheal aspirates and bronchoalveolar lavage in racehorses 2. Evaluation of the diagnostic significance of neutrophil percentage [J].

Malikides, N ;

Hughes, KJ ;

Hodgson, DR ;

Hodgson, JL .

AUSTRALIAN VETERINARY JOURNAL, 2003, 81 (11) :685-687

[8] An Official American Thoracic Society Clinical Practice Guideline: The Clinical Utility of Bronchoalveolar Lavage Cellular Analysis in Interstitial Lung Disease [J].

Meyer, Keith C. ;

Raghu, Ganesh ;

Baughman, Robert P. ;

Brown, Kevin K. ;

Costabel, Ulrich ;

du Bois, Roland M. ;

Drent, Marjolein ;

Haslam, Patricia L. ;

Kim, Dong Soon ;

Nagai, Sonoko ;

Rottoli, Paola ;

Saltini, Cesare ;

Selman, Moises ;

Strange, Charlie ;

Wood, Brent .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2012, 185 (09) :1004-1014

[9] Laboratory findings in respiratory fluids of the poorly-performing horse [J].

Richard, Eric A. ;

Fortier, Guillaume D. ;

Lekeux, Pierre M. ;

Van Erck, Emmanuelle .

VETERINARY JOURNAL, 2010, 185 (02) :115-122

[10] Induced sputum cellularity - Reference values and distribution in normal volunteers [J].

Spanevello, A ;

Confalonieri, N ;

Sulotto, F ;

Romano, F ;

Balzano, G ;

Migliori, GB ;

Bianchi, A ;

Michetti, G .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2000, 162 (03) :1172-1174

← 1 2 →