The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly

被引：8

作者：

Chen, Minghai ^{[1
]}

Yan, Chuang ^{[1
]}

Qin, Fujun ^{[1
]}

Zheng, Luping ^{[1
]}

Zhang, Xian-En ^{[2
,3
]}

机构：

[1] Chinese Acad Sci, CAS Key Lab Quantitat Engn Biol, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China

[2] Chinese Acad Sci, Shenzhen Inst Adv Technol, Fac Synthet Biol, Shenzhen 518055, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | 2021年 / 17卷 / 14期

关键词：

SARS-CoV-2; interactome; nucleocapsid protein; BiFC; VLPs; CORONAVIRUS; SIGNAL;

D O I：

10.7150/ijbs.64977

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins of SARS-CoV-2, and also specifically interacted with the putative packaging signal (PS) of SARS-CoV-2. We also demonstrated that the PS core containing PS576 RNA bears a functional PS, important for the assembly of the viral RNA into virus like particles (VLPs), and the packaging of SARS-CoV-2 RNA was N dependent.

引用

页码：3889 / 3897

页数：9

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