Molecular-cytogenetic characterization of the Vicia faba genome - heterochromatin differentiation, replication patterns and sequence localization

被引：59

作者：

Fuchs, J

Strehl, S

Brandes, A

Schweizer, D

Schubert, I ^{[1
]}

机构：

[1] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

[2] Univ Vienna, Inst Bot, Abt Cytol & Genet, A-1030 Vienna, Austria

来源：

CHROMOSOME RESEARCH | 1998年 / 6卷 / 03期

关键词：

banding and replication patterns; chromosome domains; fluorescence in situ hybridization; Vicia faba;

D O I：

10.1023/A:1009215802737

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comprehensive survey of the molecular-cytogenetic features of the Vicia faba chromosome complement (2n = 12) is given. It includes previous as well as new original data. Various Giemsa, restriction endonuclease and fluorochrome banding patterns, azacytidine-mediated segment extension, replication patterns, lateral A/T asymmetry and sequence localization data for tandemly arranged simple sequence repeats, dispersed repeats and coding sequences as well as histone acetylation patterns are considered. This allows not only to distinguish and characterize telomeres, subtelomeres, centromeres and the NOR, but also the structure of the 5S rRNA gene loci and two main types of interstitial heterochromatin. Additionally, it offers physical landmarks within euchromatic areas. Thus, the field bean genome, exemplified by the reconstructed karyotype ACB, belongs to the cytogenetically best investigated plant genomes.

引用

页码：219 / 230

页数：12

共 82 条

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