Sensitive determination of atorvastatin in human plasma by dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high-performance liquid chromatography

被引:42
作者
Taheri, Salman [1 ]
Jalali, Fahimeh [1 ]
Fattahi, Nazir [2 ]
Bahrami, Gholamreza [3 ]
机构
[1] Razi Univ, Dept Chem, Kermanshah, Iran
[2] Kermanshah Univ Med Sci, RCEDH, Kermanshah, Iran
[3] Kermanshah Univ Med Sci, Sch Pharm, Kermanshah, Iran
关键词
Atorvastatin; Blood analysis; Microextraction; Organic drop; Solidification; SOLID-PHASE MICROEXTRACTION; GAS-CHROMATOGRAPHY; ULTRAVIOLET DETECTION; MASS-SPECTROMETRY; AQUEOUS SAMPLES; STATIN THERAPY; EXTRACTION; DRUGS; WATER; ANTIDEPRESSANTS;
D O I
10.1002/jssc.201401115
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel and sensitive dispersive liquid-liquid microextraction method based on the solidification of the floating organic drop combined with high-performance liquid chromatography and ultraviolet detection was used for the determination of atorvastatine in blood serum samples. The chromatographic separation of atorvastatin was carried out using methanol as the mobile phase organic modifier. Various parameters affecting the extraction efficiency were optimized, such as the kind and volume of extraction solvent (1-undecanol) and disperser solvent (acetonitrile), pH, and the extraction time. The calibration curve was linear in the range of 0.2-6000 mu g/L of atorvastatin (r(2) = 0.995) with a limit of detection of 0.07 mu g/L. The relative standard deviation for 100 mu g/L of atorvastatin in human plasma was 8.4% (n = 4). The recoveries of plasma samples spiked with atorvastatin were in the range of 98.8-113.8%. The obtained results showed that the proposed method is fast, simple, and reliable for the determination of very low concentrations of atorvastatin in human plasma samples.
引用
收藏
页码:309 / 315
页数:7
相关论文
共 47 条
  • [1] Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography-ultraviolet detection to determination of opium alkaloids in human plasma
    Ahmadi-Jouibari, Toraj
    Fattahi, Nazir
    Shamsipur, Mojtaba
    Pirsaheb, Meghdad
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2013, 85 : 14 - 20
  • [2] Liquid chromatographic determination of atorvastatin in bulk drug, tablets, and human plasma
    Altuntas, TG
    Erk, N
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2004, 27 (01) : 83 - 93
  • [3] [Anonymous], 2013, WORLD HLTH REPORT 20
  • [4] SOLID-PHASE MICROEXTRACTION WITH THERMAL-DESORPTION USING FUSED-SILICA OPTICAL FIBERS
    ARTHUR, CL
    PAWLISZYN, J
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (19) : 2145 - 2148
  • [5] Spectrophotometric and TLC-densitometric methods for the simultaneous determination of Ezetimibe and Atorvastatin calcium
    Baghdady, Yehia Z.
    Al-Ghobashy, Medhat A.
    Abdel-Aleem, Abdel-Aziz E.
    Weshahy, Soheir A.
    [J]. JOURNAL OF ADVANCED RESEARCH, 2013, 4 (01) : 51 - 59
  • [6] Determination of atorvastatin in human serum by reversed-phase high-performance liquid chromatography with UV detection
    Bahrami, G
    Mohammadi, B
    Mirzaeei, S
    Kiani, A
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 826 (1-2): : 41 - 45
  • [7] Efficacy and safety of atorvastatin compared to pravastatin in patients with hypercholesterolemia
    Bertolini, S
    Bon, GB
    Campbell, LM
    Farnier, M
    Langan, J
    Mahla, G
    Pauciullo, P
    Sirtori, C
    Egros, F
    Fayyad, R
    Nawrocki, JW
    [J]. ATHEROSCLEROSIS, 1997, 130 (1-2) : 191 - 197
  • [8] Mild to moderate muscular symptoms with high-dosage statin therapy in hyperlipidemic patients -: The PRIMO study
    Bruckert, E
    Hayem, G
    Dejager, S
    Yau, C
    Bégaud, B
    [J]. CARDIOVASCULAR DRUGS AND THERAPY, 2005, 19 (06) : 403 - 414
  • [9] Solid-phase microextraction using poly(pyrrole) film and liquid chromatography with UV detection for analysis of antidepressants in plasma samples
    Chaves, Andrea Rodrigues
    Chiericato Junior, Glaico
    Costa Queiroz, Maria Eugema
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (07): : 587 - 593
  • [10] Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography as an efficient and sensitive technique for simultaneous determination of chloramphenicol and thiamphenicol in honey
    Chen, Huaixia
    Chen, Hui
    Ying, Jun
    Huang, Jianlin
    Liao, Lei
    [J]. ANALYTICA CHIMICA ACTA, 2009, 632 (01) : 80 - 85