The role of vimentin as a methylation biomarker for early diagnosis of cervical cancer

被引：31

作者：

Jung, Samil ^{[1
,2
]}

Yi, Lisha ^{[1
,2
]}

Kim, Jinsun ^{[1
,2
]}

Jeong, Dongjun ^{[3
]}

Oh, Taejeong ^{[4
]}

Kim, Chang-Hwan ^{[3
]}

Kim, Chang-Jin ^{[3
]}

Shin, Jin ^{[5
]}

An, Sungwhan ^{[4
]}

Lee, Myeong-Sok ^{[1
,2
]}

机构：

[1] Sookmyung Womens Univ, Div Biol Sci, Seoul 140742, South Korea

[2] Sookmyung Womens Univ, Res Ctr Womens Dis, Seoul 140742, South Korea

[3] Soonchunhyang Univ, Dept Pathol, Coll Med, Cheonan 330090, South Korea

[4] Genomictree Inc, Taejon 305811, South Korea

[5] Songdo Technopk, Inchon 406840, South Korea

来源：

MOLECULES AND CELLS | 2011年 / 31卷 / 05期

关键词：

cervical cancer; epigenetic gene regulation; methylation biomarker; VIM; HUMAN-PAPILLOMAVIRUS; DNA; EXPRESSION; MIGRATION; NEOPLASIA;

D O I：

10.1007/s10059-011-0229-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiple cytosine guanine dinucleotides (CpG island) are found in the VIM promoter region. The levels of VIM promoter methylation and VIM gene expression were investigated in 7 cervical cancer cell lines and 50 human tissue samples with a distinctive degree of malignant trans-formation. While multiple CpG sites in the VIM promoter were highly methylated in CIN III and invasive carcinoma cells, they were rarely methylated in normal cells. Our result shows that methylation in the VIM promoter appears to start from CIN I and CIN II, relatively early stages of multistep carcinogenesis. This epigenetic alteration in VIM promoter suggests the availability as a biomarker for the early diagnosis and prevention of cervical cancer. We also show that hypermethylation in the VIM promoter is responsible for transcriptional silencing of the VIM gene in cervical cancer cells. In addition, our result shows that exogenous overexpression of the VIM gene in SiHa cervical cancer cells slightly activated cell proliferation and migration as shown in soft agar colony formation and migration assays.

引用

页码：405 / 411

页数：7

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