Presence of the oral bacterium Capnocytophaga canimorsus in the tooth plaque of canines

被引:17
作者
Dilegge, S. K. [1 ]
Edgcomb, V. P. [2 ]
Leadbetter, E. R. [2 ]
机构
[1] Smith Coll, Northampton, MA 01063 USA
[2] Woods Hole Oceanog Inst, Woods Hole, MA USA
关键词
Capnocytophaga canimorsus; Canine; Tooth plaque; IDENTIFICATION; MENINGITIS; SOFTWARE; MODELS; FLORA; DOGS;
D O I
10.1016/j.vetmic.2010.12.010
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Capnocytophaga canimorsus is a potentially pathogenic microorganism when transmitted to humans from the oral cavity of canines. Although there is some knowledge about the frequency of occurrence in canines, it is uncertain whether there is a correlation between its occurrence and lifestyle, health, or breed of dog. Samples of tooth plaque from a total of 131 canines were collected, cultured on selective media, and tested using physiological and molecular analyses to help discern the presence of C. canimorsus. Phylogenetic analyses determined that 49.2% of canines sampled carried a species of Capnocytophaga and 21.7% of the canines sampled in this study carried C. canimorsus. Statistical analyses found that male dogs and those that are neutered and spayed are more likely to host Capnocytophaga species. The data also suggested that breed was a statistically significant predictor of C. canimorsus, with the smaller breeds more likely to carry the potential pathogen. In addition, three "human" species of Capnocytophaga; C ochracea, C haemolytica, and one isolate of either C. gingivalis or C. granulosa were cultured from five canines. Sixteen canines sampled carried an unidentified Capnocytophaga species, with the sequences from all isolates forming a well-defined phylogenetic clade with 100% bootstrap support that may well represent a new species of Capnocytophaga. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:437 / 445
页数:9
相关论文
共 27 条
[1]   INVESTIGATIONS ON METABOLITES OF MICROORGANISMS .25. ON THE ORIGIN OF THE BRANCHED ALKYL SUBSTITUENTS ON RING-B OF FLEXIRUBIN-TYPE PIGMENTS [J].
ACHENBACH, H ;
BOTTGERVETTER, A ;
FAUTZ, E ;
REICHENBACH, H .
ARCHIVES OF MICROBIOLOGY, 1982, 132 (03) :241-244
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]  
BAILIE WE, 1978, J CLIN MICROBIOL, V7, P223
[4]   Capnocytophaga canimorsus in the oral flora of dogs and cats [J].
Blanche, P ;
Bloch, E ;
Sicard, D .
JOURNAL OF INFECTION, 1998, 36 (01) :134-134
[5]   Molecular identification of Capnocytophaga spp. via 16S rRNA PCR-restriction fragment length polymorphism analysis [J].
Ciantar, M ;
Newman, HN ;
Wilson, M ;
Spratt, DA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (04) :1894-1901
[6]   The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy [J].
Cole, JR ;
Chai, B ;
Marsh, TL ;
Farris, RJ ;
Wang, Q ;
Kulam, SA ;
Chandra, S ;
McGarrell, DM ;
Schmidt, TM ;
Garrity, GM ;
Tiedje, JM .
NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :442-443
[7]   Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].
Ewing, B ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :186-194
[8]   Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].
Ewing, B ;
Hillier, L ;
Wendl, MC ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :175-185
[9]   Capnocytophaga canimorsus [J].
Gaastra, Wim ;
Lipman, Len J. A. .
VETERINARY MICROBIOLOGY, 2010, 140 (3-4) :339-346
[10]   Cross-Kingdom Amplification Using Bacteria-Specific Primers: Complications for Studies of Coral Microbial Ecology [J].
Galkiewicz, Julia P. ;
Kellogg, Christina A. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (24) :7828-7831