Daily variations in oligosaccharides of human milk determined by microfluidic chips and mass spectrometry

被引：85

作者：

Ninonuevo, Milady R. ^{[1
]}

Perkins, Patrick D. ^{[2
]}

Francis, Jimi ^{[3
,4
]}

Lamotte, LaTasha A. ^{[1
]}

LoCascio, Riccardo G. ^{[5
]}

Freeman, Samara L. ^{[6
]}

Mills, David A.

German, J. Bruce ^{[6
]}

Grimm, Rudolf

Lebrilla, Carlito B. ^{[1
,7
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Agilent Technol, Santa Clara, CA 95052 USA

[3] Univ Nevada, Dept Biochem, Reno, NV 89557 USA

[4] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA

[5] Univ Calif Davis, Microbiol Grad Grp, Davis, CA 95616 USA

[6] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA

[7] Univ Calif Davis, Dept Biochem, Sch Med, Davis, CA 95616 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 02期

关键词：

oligosaccharicles; human milk; HPLC-Chip/MS technology; lactation; prebiotics; bifidobacteria; microfluidic; mass spectrometry;

D O I：

10.1021/jf071972u

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Human milk is a complex biological fluid that provides not only primary nourishment for infants but also protection against pathogens and influences their metabolic, immunologic, and even cognitive development. The presence of oligosaccharides in remarkable abundance in human milk has been associated to provide diverse biological functions including directing the development of an infant's intestinal microflora and immune system. Recent advances in analytical tools offer invaluable insights in understanding the specific functions and health benefits these biomolecules impart to infants. Oligosaccharides in human milk samples obtained from five different individual donors over the course of a 3 month lactation period were isolated and analyzed using HPLC-Chip/TOF-MS technology. The levels and compositions of oligosaccharides in human milk were investigated from five individual donors. Comparison of HPLC-Chip/TOF-MS oligosaccharides profiles revealed heterogeneity among multiple individuals with no significant variations at different stages of lactation within individual donors.

引用

页码：618 / 626

页数：9

共 29 条

[1]

ASAKUMA S, 2007, IN PRESS EUR J CLIN

[2] Recent advances on structure, metabolism, and function of human milk oligosaccharides [J].