E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly

被引：42

作者：

Gosavi, Prajakta ^{[1
]}

Kundu, Samrat T. ^{[1
]}

Khapare, Nileema ^{[1
]}

Sehgal, Lalit ^{[1
]}

Karkhanis, Mansi S. ^{[1
]}

Dalal, Sorab N. ^{[1
]}

机构：

[1] Tata Mem Hosp, Adv Ctr Treatment Res & Educ Canc, Kharghar Node 410210, Navi Mumbai, India

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2011年 / 68卷 / 08期

关键词：

Plakophilin3; Desmosome; Plakoglobin; INTERMEDIATE-FILAMENTS; EPITHELIAL-CELLS; BETA-CATENIN; ADHESION; PROTEIN; PLAQUE; DESMOPLAKIN; MAINTENANCE; JUNCTIONS; BINDING;

D O I：

10.1007/s00018-010-0531-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A decrease in the levels of the desmosomal plaque protein, plakophilin3 (PKP3), leads to a decrease in desmosome size and cell-cell adhesion. To test the hypothesis that PKP3 is required for desmosome formation, the recruitment of desmosomal components to the cell surface was studied in the PKP3 knockdown clones. The PKP3 knockdown clones showed decreased cell border staining for multiple desmosomal proteins, when compared to vector controls, and did not form desmosomes in a calcium switch assay. Further analysis demonstrated that PKP3, plakoglobin (PG) and E-cadherin are present at the cell border at low concentrations of calcium. Loss of either PG or E-cadherin led to a decrease in the levels of PKP3 and other desmosomal proteins at the cell border. The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to initiate desmosome formation.

引用

页码：1439 / 1454

页数：16

共 47 条

[1] Plakophilin 2:: a critical scaffold for PKCα that regulates intercellular junction assembly [J].

Bass-Zubek, Amanda E. ;

Hobbs, Ryan P. ;

Amargo, Evangeline V. ;

Garcia, Nicholas J. ;

Hsieh, Sherry N. ;

Chen, Xinyu ;

Wahl, James K., III ;

Denning, Mitchell F. ;

Green, Kathleen J. .

JOURNAL OF CELL BIOLOGY, 2008, 181 (04) :605-613

[2]

BASSZUBEK AE, 2009, CURR OPIN CELL BIOL, V181, P605

[3] Defining desmosomal plakophilin-3 interactions [J].

Bonné, S ;

Gilbert, B ;

Hatzfeld, M ;

Chen, X ;

Green, KJ ;

van Roy, F .

JOURNAL OF CELL BIOLOGY, 2003, 161 (02) :403-416

[4]

Bonné S, 1999, J CELL SCI, V112, P2265

[5]

Bornslaeger EA, 2001, J CELL SCI, V114, P727

[6] Depletion of E-cadherin disrupts establishment but not maintenance of cell junctions in Madin-Darby canine kidney epithelial cells [J].

Capaldo, Christopher T. ;

Macara, Ian G. .

MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (01) :189-200

[7] Protein binding and functional characterization of plakophilin 2 -: Evidence for its diverse roles in desmosomes and β-catenin signaling [J].

Chen, XY ;

Bonné, S ;

Hatzfeld, M ;

van Roy, F ;

Green, KJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (12) :10512-10522

[8]

Dalal SN, 1999, MOL CELL BIOL, V19, P4465

[9] Discriminating roles of desmosomal cadherins: Beyond desmosomal adhesion [J].

Dusek, Rachel L. ;

Godsel, Lisa M. ;

Green, Kathleen J. .

JOURNAL OF DERMATOLOGICAL SCIENCE, 2007, 45 (01) :7-21

[10] PAR1b promotes cell-cell adhesion and inhibits dishevelled-mediated transformation of Madin-Darby canine kidney cell [J].

Elbert, Maya ;

Cohen, David ;

Musch, Anne .

MOLECULAR BIOLOGY OF THE CELL, 2006, 17 (08) :3345-3355

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