Nodulation factor receptor kinase 1α controls nodule organ number in soybean (Glycine max L. Merr)

被引:106
作者
Indrasumunar, Arief [1 ,2 ]
Searle, Iain [3 ,4 ]
Lin, Meng-Han [1 ]
Kereszt, Attila [1 ,5 ]
Men, Artem [1 ,6 ]
Carroll, Bernard J. [1 ,3 ]
Gresshoff, Peter M. [1 ]
机构
[1] Univ Queensland, ARC Ctr Excellence Integrat Legume Res, St Lucia, Qld 4072, Australia
[2] Indonesian Ctr Agr Biotechnol & Genet Resources R, Bogor 16111, Indonesia
[3] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld 4072, Australia
[4] Australian Natl Univ, Sch Biochem & Mol Biol, Canberra, ACT 2601, Australia
[5] Human Biotechnol & Bioenergy, Inst Plant Genom, Szeged, Hungary
[6] Australian Genome Res Facil, Brisbane, Qld, Australia
基金
澳大利亚研究理事会;
关键词
gene duplication; Glycine max L; Merr; LysM receptor kinase; nodulation; soil acidity;
D O I
10.1111/j.1365-313X.2010.04398.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
P>Two allelic non-nodulating mutants, nod49 and rj1, were characterized using map-based cloning and candidate gene approaches, and genetic complementation. From our results we propose two highly related lipo-oligochitin LysM-type receptor kinase genes (GmNFR1 alpha and GmNFR1 beta) as putative Nod factor receptor components in soybean. Both mutants contained frameshift mutations in GmNFR1 alpha that would yield protein truncations. Both mutants contained a seemingly functional GmNFR1 beta homeologue, characterized by a 374-bp deletion in intron 6 and 20-100 times lower transcript levels than GmNFR1 alpha, yet both mutants were unable to form nodules. Mutations in GmNFR1 beta within other genotypes had no defects in nodulation, showing that GmNFR1 beta was redundant. Transgenic overexpression of GmNFR1 alpha, but not of GmNFR1 beta, increased nodule number per plant, plant nitrogen content and the ability to form nodules with restrictive, ultra-low Bradyrhizobium japonicum titres in transgenic roots of both nod49 and rj1. GmNFR1 alpha overexpressing roots also formed nodules in nodulation-restrictive acid soil (pH 4.7). Our results show that: (i) NFR1 alpha expression controls nodule number in soybean, and (ii) acid soil tolerance for nodulation and suppression of nodulation deficiency at low titre can be achieved by overexpression of GmNFR1 alpha.
引用
收藏
页码:39 / 50
页数:12
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