The contribution of adenines in the catalytic core of 10-23 DNAzyme improved by the 6-amino group modifications

被引：11

作者：

Zhu, Junfei ^{[1
]}

Li, Zhiwen ^{[1
]}

Wang, Qi ^{[2
,3
]}

Liu, Yang ^{[4
]}

He, Junlin ^{[2
]}

机构：

[1] Guizhou Univ, Coll Life Sci, Guiyang 550025, Peoples R China

[2] Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China

[3] Hununbeier Peoples Hosp, Hununbeier 021008, Peoples R China

[4] Guangxi Med Univ, Nanning 530021, Peoples R China

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2016年 / 26卷 / 18期

基金：

中国国家自然科学基金;

关键词：

10-23; DNAzyme; Amino group; Imidazolyl group; Chemical modification; Adenine; ACID-BASE CATALYSIS; CHEMICAL-MODIFICATION; DNA; RNA; CLEAVAGE; SEQUENCE; APTAMER; SIRNA; THERAPEUTICS; CONSERVATION;

D O I：

10.1016/j.bmcl.2016.07.076

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In the catalytic core of 10-23 DNAzyme, its five adenine residues are moderate conservative, but with highly conserved functional groups like 6-amino group and 7-nitrogen atom. It is this critical conservation that these two groups could be modified for better contribution. With 20-deoxyadenosine analogues, several functional groups were introduced at the 6-amino group of the five adenine residues. 3-Aminopropyl substituent at 6-amino group of A15 resulted in a five-fold increase of k(obs). More efficient DNAzymes are expected by delicate design of the linkage and the external functional groups for this 6-amino group of A15. With this modification approach, other functional groups or residues could be optimized for 10-23 DNAzyme. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：4462 / 4465

页数：4

共 19 条

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He, Junlin
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ACTA PHARMACEUTICA SINICA B, 2012, 2 (01) : 28 - 31
[2] A new Pb2+-specific DNAzyme by revisiting the catalytic core of 10-23 DNAzyme
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[3] 2′-Functional group of adenosine in 10-23 DNAzyme promotes catalytic activity
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BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2020, 30 (04)
[4] Incorporation of amino acid modified nucleosides into catalytic core of deoxyribozyme "10-23"
Smuga, D
Fratczak, L
Sochacka, E
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[5] Breaking the conservation of guanine residues in the catalytic loop of 10-23 DNAzyme by position-specific nucleobase modifications for rate enhancement
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Liu, Gaofeng
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[6] Sequence requirements in the catalytic core of the "10-23" DNA enzyme
Zaborowska, Z
Fürste, JP
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[8] Studies on the preferred uracil-adenine base pair at the cleavage site of 10-23 DNAzyme by functional group modifications on adenine
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[9] A combination of the modified catalytic core and conjugation of 3′-inverted deoxythymidine for a more efficient and nuclease-resistant 10-23 DNAzyme
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BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2022, 62
[10] Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
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MOLECULES, 2017, 22 (07):

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