Position-sensitive scanning fluorescence correlation spectroscopy

被引：1
|
作者：
Skinner, JP [1 ]
Chen, Y [1 ]
Müller, JD [1 ]
机构：
[1] Univ Minnesota, Sch Phys & Astron, Minneapolis, MN 55455 USA
来源：
Multiphoton Microscopy in the Biomedical Sciences V | 2005年 / 5700卷
关键词：
fluorescence fluctuation spectroscopy; two-photon microscopy; scanning microscopy; diffusion; transport processes; fluorescence correlation spectroscopy; AGGREGATION;
D O I：
10.1117/12.600823
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Most fluorescence fluctuation experiments use a stationary laser beam to illuminate a small sample volume and analyze the temporal behavior of the fluorescence fluctuations within the stationary observation volume. Scanning of the laser beam in a circular pattern collects the fluorescence signal from a moving observation volume. The fluctuations contain now information about temporal and spatial proper-ties of the sample. Synchronization between beam scanning and data collection allows us to evaluate the fluctuations for every position along the scanned trajectory. We present the theory of position-sensitive scanning fluorescence fluctuation spectroscopy and experimentally verify the theory. This technique is useful for detecting and characterizing directed transport processes in the presence of diffusion.
引用
收藏
页码：118 / 127
页数：10
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