Detection of Japanese yam mosaic virus by RT-LAMP

被引：158

作者：

Fukuta, S ^{[1
]}

Iida, T ^{[1
]}

Mizukami, Y ^{[1
]}

Ishida, A ^{[1
]}

Ueda, J ^{[1
]}

Kanbe, M ^{[1
]}

Ishimoto, Y ^{[1
]}

机构：

[1] Aichi Ken Agr Res Ctr, Aichi 4801193, Japan

来源：

ARCHIVES OF VIROLOGY | 2003年 / 148卷 / 09期

关键词：

D O I：

10.1007/s00705-003-0134-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid and simple procedure is described to detect the genomic RNA molecule of Japanese yam mosaic potyvirus (JYMV). This method, named RT-LAMP, allows direct detection of RNA from infected plants without careful RNA extraction, rapid thermal cycling and gel electrophoresis. RT-LAMP was successfully applied to leaves, propagules and roots of Japanese yam infected with JYMV One of the characteristics of the RT-LAMP method is its ability to synthesize an extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophospate ion, is produced yielding a white precipitate of magnesium pyrophosphate in the reaction mixture. The presence or absence of this white precipitate allows easy detection of the amplification of JYMV genomic RNA without gel electrophoresis.

引用

页码：1713 / 1720

页数：8

共 16 条

[11] Loop-mediated isothermal amplification of DNA [J].