Human T-cell lymphotropic virus type 1 Gag indeterminate Western blot patterns in Central Africa:: Relationship to Plasmodium falciparum infection

被引:55
|
作者
Mahieux, R
Horal, P
Mauclère, P
Mercereau-Puijalon, O
Guillotte, M
Meertens, L
Murphy, E
Gessain, A
机构
[1] Inst Pasteur, Unite Epidemiol Virus Oncogenes, Paris, France
[2] Inst Pasteur, CNRS, URA 1960, Unite Immunol Mol Parasites, Paris, France
[3] Univ Gothenburg, Dept Clin Virol, Gothenburg, Sweden
[4] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Dept Med, San Francisco, CA USA
[6] Univ Calif San Francisco, Dept Epidemiol Biostat, San Francisco, CA 94143 USA
关键词
D O I
10.1128/JCM.38.11.4049-4057.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To gain insight on the significance of human T-cell lymphotropic virus type I (HTLV-1) indeterminate serological reactivities, we studied villagers of South Cameroon, focusing on a frequent and specific HTLV-1 Gag indeterminate profile (HGIP) pattern (gag p19, p26, p28, and p30 without p24 or Env gp21 and gp46), Among the 102 sera studied, 29 from all age groups had a stable HGIP pattern over a period of 4 years. There was no epidemiological evidence for sexual or vertical transmission of HGIP. Seventy-five percent of HGIP sera reacted positively on MT2 HTLV-1-infected cells by immunofluorescence assay. However, we could not isolate any HTLV-1 virus or detect the presence of p19 Gag protein in cultures of peripheral blood mononuclear cells obtained from individuals with strong HGIP reactivity. PCR experiments conducted with primers for HTLV-1 and HTLV-2 (HTLV-1/2 primers) encompassing different regions of the virus did not yield HTLV-1/2 proviral sequences from individuals with HGIP, Using 11 peptides corresponding to HTLV-1 or HTLV-2 immunodominant B epitopes in an enzyme-linked immunosorbent assay, one epitope corresponding to the Gag p19 carboxyl terminus was identified in 75% of HGIP sera, while it was recognized by only 41% of confirmed HTLV-1-positive sera. A positive correlation between HTLV-1 optical density values and titers of antibody to Plasmodium falciparum was also demonstrated. Finally, passage of sera through a P. falciparum-infected erythrocyte-coupled column was shown to specifically abrogate HGIP reactivity but not the HTLV-1 pattern, suggesting the existence of cross-reactivity between HTLV-1 Gag proteins and malaria-derived antigens, These data suggest that in Central Africa, this frequent and specific Western blot is not caused by HTLV-1 infection but could instead be associated with P. falciparum infection.
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页码:4049 / 4057
页数:9
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