Coilin interacts with Ku proteins and inhibits in vitro non-homologous DNA end joining

被引：25
作者：
Velma, Venkatramreddy [1 ]
Carrero, Zunamys I. [1 ]
Cosman, Angela M. [1 ]
Hebert, Michael D. [1 ]
机构：
[1] Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA
来源：
FEBS LETTERS | 2010年 / 584卷 / 23期
关键词：
Coilin; Ku70; Ku80; NHEJ; CAJAL BODIES; BODY FORMATION; SMN; PHOSPHORYLATION; INTERPHASE; SNRNPS; CELLS;
D O I：
10.1016/j.febslet.2010.11.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coilin is a nuclear protein that plays a role in Cajal body formation. The function of nucleoplasmic coilin is unknown. Here we report that coilin interacts with Ku70 and Ku80, which are major players in the DNA repair process. Ku proteins compete with SMN and SmB` proteins for coilin interaction sites. The binding domain on coilin for Ku proteins cannot be localized to one discrete region, and only full-length coilin is capable of inhibiting in vitro non-homologous DNA end joining (NHEJ). Since Ku proteins do not accumulate in CBs, these findings suggest that nucleoplasmic coilin participates in the regulation of DNA repair. Structured summary: MINT-8052983: coilin (uniprotkb:P38432) physically interacts (MI:0915) with SmB0 (uniprotkb:P14678) by pull down (MI:0096) MINT-8052941: coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku70 (uniprotkb:P12956) by competition binding (MI:0405) MINT-8052765: coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by pull down (MI:0096) MINT-8052971: coilin (uniprotkb:P38432) physically interacts (MI:0915) with SMN (uniprotkb:Q16637) by pull down (MI:0096) MINT-8052957: coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by competition binding (MI:0405) MINT-8052894, MINT-8052908:coilin (uniprotkb:P38432) binds (MI:0407) to Ku80 (uniprotkb:P13010) by pull down (MI:0096) MINT-8052804: coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku80 (uniprotkb:P13010) by anti bait coimmunoprecipitation (MI:0006) MINT-8052925: coilin (uniprotkb:P38432) binds (MI:0407) to Ku70 (uniprotkb:P12956) by pull down (MI:0096) MINT-8052786: Ku80 (uniprotkb:P13010) physically interacts (MI:0914) with coilin (uniprotkb:P38432) and Ku70 (uniprotkb:P12956) by anti bait coimmunoprecipitation (MI:0006) MINT-8052776: coilin (uniprotkb:P38432) physically interacts (MI:0915) with Ku70 (uniprotkb:P12956) by pull down (MI:0096) (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：4735 / 4739
页数：5
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