Changes in aurantio-obtusin and glucoaurantio-obtusin content in Cassiae Semen via treatment with a crude enzyme extract from Aspergillus usamii

被引：0

作者：

Hur, Jong-Moon

Kwon, Soon-Ho

So, Jae-Hyun

Jun, Mira

Kang, Young-Hwa

Lee, Yu-Mi

Lee, Kyung-Bok

Rhee, In-Koo

Lee, Moon-Soon

Song, Kyung-Sik ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Coll Agr & Life Sci, Sch Appl Biosci, Taegu 702701, South Korea

[2] Dong A Univ, Div Food Sci, Pusan 604714, South Korea

[3] Kyungpook Natl Univ, Coll Nat Sci, Dept Genet Engn, Taegu 702701, South Korea

[4] Konyang Univ, Coll Med, Taejon 302718, South Korea

[5] Nat Inst Environm Res, Chem Assessment Dept, Chem Safety & Accidental Prevent Div, Inchon 404708, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 17卷 / 11期

关键词：

Cassiae Semen; aurantio-obtusin; glucoaurantio-obtusin; Aspergillus usamii; processes; enzyme treatment;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level (133.58 +/- 0.39 mu g/mg extract) after being incubated for 50 min at 37 degrees C, whereas the inactivated crude enzyme-treated control remained unchanged (54.13 +/- 1.33 mu g/mg). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third (51.09 +/- 1.63 mu g/mg) of the untreated control (143.19 +/- 2.12 mu g/mg), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.

引用

页码：1894 / 1897

页数：4