An Open-Source Simulator for Exploring HPLC Theory

被引：0

作者：

Abate-Pella, Daniel ^{[1
]}

Stoll, Dwight R. ^{[2
]}

Carr, Peter W. ^{[3
]}

Boswell, Paul G. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Hort Sci, St Paul, MN 55105 USA

[2] Gustavus Adolphus Coll, Dept Chem, St Peter, MN 56082 USA

[3] Univ Minnesota, Dept Chem, St Paul, MN USA

来源：

LC GC NORTH AMERICA | 2015年 / 33卷 / 03期

基金：

美国国家卫生研究院;

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; GRADIENT ELUTION;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available,for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation? How much does extracolumn tubing affect a separation?

引用

页码：200 / 207

页数：8

共 12 条

[1] An Advanced, Interactive, High-Performance Liquid Chromatography Simulator and Instructor Resources [J].

Boswell, Paul G. ;

Stoll, Dwight R. ;

Carr, Peter W. ;

Nagel, Megan L. ;

Vitha, Mark F. ;

Mabbott, Gary A. .

JOURNAL OF CHEMICAL EDUCATION, 2013, 90 (02) :198-202

[2] USING COMPUTERS TO REPLACE SOME HPLC LABORATORY WORK [J].

BOWATER, IC ;

MCWILLIAM, IG .

JOURNAL OF CHEMICAL EDUCATION, 1994, 71 (08) :674-678

[3] Maintaining fixed band spacing when changing column dimensions in gradient elution [J].

Dolan, JW ;

Snyder, LR .

JOURNAL OF CHROMATOGRAPHY A, 1998, 799 (1-2) :21-34

[4]

Guillarme D, 2008, LC GC EUR, V21, P322

[5] Training software for high-performance liquid chromatography [J].

Reijenga, JC .

JOURNAL OF CHROMATOGRAPHY A, 2000, 903 (1-2) :41-48

[6]

Rittenhouse R. C., 1995, J CHEM EDUC, V72, P1086

[7] JOURNAL OF CHEMICAL EDUCATION - SOFTWARE - HPLC - A COMPUTER-SIMULATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

RITTENHOUSE, RC .

JOURNAL OF CHEMICAL EDUCATION, 1988, 65 (12) :1050-1050

[8] A practical approach to transferring linear gradient elution methods [J].

Schellinger, AP ;

Carr, PW .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1077 (02) :110-119

[9] Optimizing chromatographic separation: An experiment using an HPLC simulator [J].

Shalliker, R. A. ;

Kayillo, S. ;

Dennis, G. R. .

JOURNAL OF CHEMICAL EDUCATION, 2008, 85 (09) :1265-1268

[10]

Snyder L.R., 2010, INTRO MODERN LIQUID, P405

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