A comparison of the expression and properties of Apaf-1 and Apaf-1L

被引:8
|
作者
Walke, DW [1 ]
Morgan, JI [1 ]
机构
[1] St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USA
关键词
RNase protection; two-hybrid; dimerization; cell death; procapase-9;
D O I
10.1016/S0006-8993(00)02916-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Apaf-1 is a mammalian homolog of CED-1 that regulates cell death by participating in a ternary complex with cytochrome c, and procaspase-9. In the case of CED-4, two splice variants exist. The smaller (CED-4S) is proapoptotic while the larger (CED-4L) contains a short in-frame insert and is anti-apoptotic. We cloned a murine variant of apaf-1, termed apaf-1L. which contains an eleven amino acid insert similar to a recently described human apaf-1L clone. apaf-1 and apaf-1L have similar distributions in adult and fetal tissues. although apaf-1L transcripts are more abundant. Apaf-1L. undergoes homomerization and heteromerization with Apaf-1 in yeast. Apaf-1L also binds to caspase-9 and a dominant-negative isoform of caspase-9. Unlike CED-4. neither Apaf-1 variant was lethal in yeast. However, both Apaf-1 and Apaf-1L elicit cell death when cotransfected with caspase-9 into 293 EBNA cells. Although Apaf-1L was more potent than Apaf-1, their biological properties were qualitatively similar. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:73 / 81
页数:9
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