Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase

被引：135

作者：

Chan, Kai Xun ^{[1
]}

Mabbitt, Peter D. ^{[2
]}

Phua, Su Yin ^{[1
]}

Mueller, Jonathan W. ^{[3
,4
]}

Nisar, Nazia ^{[1
]}

Gigolashvili, Tamara ^{[5
]}

Stroeher, Elke ^{[6
]}

Grassl, Julia ^{[6
]}

Arlt, Wiebke ^{[3
,4
]}

Estavillo, Gonzalo M. ^{[1
,7
]}

Jackson, Colin J.

Pogson, Barry J. ^{[1
]}

机构：

[1] Australian Natl Univ, Res Sch Biol, Australian Res Council Ctr Excellence Plant Energ, Acton, ACT 2601, Australia

[2] Australian Natl Univ, Res Sch Chem, Acton, ACT 2601, Australia

[3] Univ Birmingham, Inst Metab & Syst Res, Birmingham B15 2TT, W Midlands, England

[4] Birmingham Hlth Partners, Ctr Endocrinol Diabet & Metab, Birmingham B15 2TH, W Midlands, England

[5] Univ Cologne, Cluster Excellence Plant Sci, Inst Bot, D-50674 Cologne, Germany

[6] Univ Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, Australia

[7] Commonwealth Sci & Ind Res Org Agr, Canberra, ACT 2601, Australia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2016年 / 113卷 / 31期

基金：

英国惠康基金; 澳大利亚研究理事会;

关键词：

retrograde signaling; redox regulation; stress sensing; chloroplast; drought stress; NORMAL-MODE ANALYSIS; REDOX REGULATION; ALLOSTERIC INHIBITION; SINGLET OXYGEN; ARABIDOPSIS; PROTEIN; DROUGHT; GLUTATHIONE; EXPRESSION; MECHANISM;

D O I：

10.1073/pnas.1604936113

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intracellular signaling during oxidative stress is complex, with organelle-to-nucleus retrograde communication pathways ill-defined or incomplete. Here we identify the 3'-phosphoadenosine 5'-phosphate (PAP) phosphatase SAL1 as a previously unidentified and conserved oxidative stress sensor in plant chloroplasts. Arabidopsis thaliana SAL1 (AtSAL1) senses changes in photosynthetic redox poise, hydrogen peroxide, and superoxide concentrations in chloroplasts via redox regulatory mechanisms. AtSAL1 phosphatase activity is suppressed by dimerization, intramolecular disulfide formation, and glutathionylation, allowing accumulation of its substrate, PAP, a chloroplast stress retrograde signal that regulates expression of plastid redox associated nuclear genes (PRANGs). This redox regulation of SAL1 for activation of chloroplast signaling is conserved in the plant kingdom, and the plant protein has evolved enhanced redox sensitivity compared with its yeast ortholog. Our results indicate that in addition to sulfur metabolism, SAL1 orthologs have evolved secondary functions in oxidative stress sensing in the plant kingdom.

引用

页码：E4567 / E4576

页数：10

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