Immunomodulation of RAW264.7 macrophages by GLIS, a proteopolysaccharide from Ganoderma lucidum

被引：72
作者：
Ji, Zhe
Tang, Qingjiu [1 ]
Zhang, Jinsong
Yang, Yan
Jia, Wei
Pan, Yingjie
机构：
[1] Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China
[2] Nanjing Agr Univ, Key Lab Microbiol Engn Agr Environm, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
[3] Shanghai Fisheries Univ, Shanghai 200090, Peoples R China
来源：
JOURNAL OF ETHNOPHARMACOLOGY | 2007年 / 112卷 / 03期
关键词：
cytokines; Ganoderma lucidum (GL); immunoactivation;
D O I：
10.1016/j.jep.2007.03.035
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The immunomodulatory effect of Ganoderma lucidum immunomodulating substance (GLIS) on macrophages has been investigated as part of on-going research into the anti-cancer properties of Ganoderma lucidum. Proliferation of bone marrow macrophages (BMMs) was enhanced by GLIS in a dose-dependent manner. Microscopic examination revealed that numerous GLIS-treated RAW264.7 macrophages were enlarged and formed pseudopodia. Exposure of RAW264.7 macrophages to GLIS resulted in significant increases in NO production, induction of cellular respiratory burst activity, and increased levels of IL-1 beta, IL-12p35 and IL-12p40 gene expression. Our data indicate that GLIS activates the immune system by modulating cytokine production. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
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收藏
页码：445 / 450
页数：6
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