Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)

被引：72

作者：

Liu, Aihong ^{[1
]}

Guan, Guiquan ^{[1
]}

Liu, Zhijie ^{[1
]}

Liu, Junlong ^{[1
]}

Leblanc, Neil ^{[2
]}

Li, Youquan ^{[1
]}

Gao, Jinliang ^{[1
]}

Ma, Milin ^{[1
]}

Niu, Qinli ^{[1
]}

Ren, Qiaoyun ^{[1
]}

Bai, Qi ^{[1
]}

Yin, Hong ^{[1
]}

Luo, Jianxun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Peoples R China

[2] World Org Anim Hlth, Collaborating Ctr Biotechnol Based Diag Infect Di, Natl Vet Inst SVA, Dept Virol Immunobiol & Parasitol, SE-75689 Uppsala, Sweden

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 126卷 / 04期

关键词：

Theileria sergenti; Theileria sinensis; MPSP gene; PCR; Detection; Differentiation; RED-BLOOD-CELLS; SEQUENCE-ANALYSIS; BOVINE THEILERIA; GENE; BUFFELI;

D O I：

10.1016/j.exppara.2010.05.024

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Theileria sergenti and Theileria sinensis are closely related members of benign Theileria species found in cattle and yaks in China. They are morphologically indistinguishable. A polymerase chain reaction (PCR) targeting major piroplasm surface protein of T. sergenti and T. sinensis was developed in this study. The newly developed oligonucleotide primer set was able to specifically amplify the DNA of T. sinensis and in conjunction with primers for T. sergenti and these two species could be detected and distinguished. Specificity testing also revealed that there was no cross-reaction with the other tick-borne diseases Theileria annulata, Babesia ovata, Ana plasma marginate as well as bovine white blood cells. Phylogenetic analysis based on the MPSP gene sequences confirmed the specificity of PCR assays. The sensitivity of the methods was 0.1 pg DNA for the T. sergenti PCR and 1 pg DNA for T. sinensis PCR. Two hundred and thirty-six field blood samples from of cattle and yaks were collected from five different geographical regions in China where benign Theileria species have been found. T. sergenti was found in all five provinces but was absent from one county in Gansu Province. T. sinensis was only found in Gansu Province. In both counties in Gansu where the parasites co-existed, mixed infections were detected. Our results indicate that the PCR methods developed in this study are suitable for the detection and differentiation of T. sergenti and T. sinensis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：476 / 481

页数：6

共 31 条

[1] DISCRIMINATION BETWEEN 6 SPECIES OF THEILERIA USING OLIGONUCLEOTIDE PROBES WHICH DETECT SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCES [J].