The Wild-Type Hepatitis C Virus Core Inhibits Initiation of Antigen-Specific T- and B-Cell Immune Responses in BALB/c Mice

被引：14
|
作者：
Zhu, Wenbo [1 ,4 ]
Chang, Yanzi [2 ]
Wu, Chunchen [1 ]
Han, Qingxia [1 ]
Pei, Rongjuan [1 ,4 ]
Lu, Mengji [1 ,2 ,3 ]
Chen, Xinwen [1 ]
机构：
[1] Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Pathogen Biol, Div Microbiol, Wuhan 430074, Peoples R China
[3] Univ Hosp Essen, Inst Virol, D-45122 Essen, Germany
[4] Chinese Acad Sci, Grad Univ, Beijing 10039, Peoples R China
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2010年 / 17卷 / 07期
关键词：
DENDRITIC CELLS; SUBCELLULAR-LOCALIZATION; LYMPHOCYTE RESPONSE; ENVELOPE PROTEIN; IN-VIVO; INFECTION; IMMUNIZATION; GENE; ACTIVATION; SUPPRESSION;
D O I：
10.1128/CVI.00490-09
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In this study, the effects of wild-type and deletion mutant hepatitis C virus (HCV) core proteins on the induction of immune responses in BALB/c mice were assessed. p2HA-C145-S23, encoding a core protein with the C-terminal 46 amino acids truncated, significantly produced stronger antibody and cellular responses than p2HA-C191-S23. The induction of immune responses by p2HA-C145-S23 was dose dependent. However, increasing the doses or repeated administration did not enhance immune responses by the wild-type core protein. In addition, p2HA-C191-S23 was apparently able to interfere with the priming of specific immune responses by p2HA-C145-S23 when the two were coadministered. These results demonstrated that the wild-type HCV core protein itself could inhibit the priming of immune responses in the course of a DNA vaccination, whereas the truncated HCV core protein could provide potential applications for the development of DNA-and peptide-based HCV vaccines.
引用
收藏
页码：1139 / 1147
页数：9
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