Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus

被引：47

作者：

Stratford, R ^{[1
]}

Douce, G ^{[1
]}

Zhang-Barber, L ^{[1
]}

Fairweather, N ^{[1
]}

Eskola, J ^{[1
]}

Dougan, G ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England

来源：

VACCINE | 2000年 / 19卷 / 7-8期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

DNA vaccines; tetanus; codon usage; murine model;

D O I：

10.1016/S0264-410X(00)00246-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/ stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：810 / 815

页数：6

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