Sedimentation field flow fractionation purification of immature neural cells from a human tumor neuroblastoma cell line

被引：30
作者：
Lautrette, C
Cardot, PJP
Vermot-Desroches, C
Wijdenes, J
Jauberteau, MO
Battu, S
机构：
[1] Univ Limoges, Lab Chim Analyt & Bromatol, Fac Pharm, F-87025 Limoges, France
[2] Univ Limoges, Lab Neoroimmunol, CNRS, UMR 6101,Fac Med, F-87025 Limoges, France
[3] Diaclone, F-25020 Besancon, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 791卷 / 1-2期
关键词：
sedimentation field flow fractionation; cell sorting; neurons;
D O I：
10.1016/S1570-0232(03)00229-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of stem cells for therapeutic applications is now an important objective for the future. Stem cell preparation is difficult and time-consuming depending on the origin of cells. Sedimentation field flow fractionation (SdFFF) is an effective tool for cell separation, respecting integrity and viability. We used the human neuroblastic SH-SY5Y clone of the SK-N-SH cell line as a source of immature neural cells. Our results demonstrated that by using SdFFF cell sorter under strictly defined conditions, and immunological cell characterization, we are now able to provide, in less than 15 min, a sterile, viable, useable and purified immature neural cell fraction without inducting cell differentiation. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：149 / 160
页数：12
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