The effect of pretreatments on surfactin production from potato process effluent by Bacillus subtilis

被引：15

作者：

Thompson, DN ^{[1
]}

Fox, SL ^{[1
]}

Bala, GA ^{[1
]}

机构：

[1] Idaho Natl Engn & Environm Lab, Dept Biotechnol, Idaho Falls, ID 83415 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2001年 / 91-3卷 / 1-9期

关键词：

Bacillus subtilis; biosurfactant; surfactin; alternate feedstock; enhanced oil recovery;

D O I：

10.1385/ABAB:91-93:1-9:487

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pretreatments of low-solids potato process effluent were tested for their potential to increase surfactin Yield. Pretreatments included heat, removal of starch particulates, and acid hydrolysis. Elimination of contaminating vegetative cells was necessary for surfactin production. After autoclaving, 0.40 g/L of surfactin was produced from the effluent in 72 h, vs 0.24 g/L in the purified potato starch control. However, surfactin yields per carbon consumed were 76% lower from process effluent. Removal of starch particulates had little effect on the culture. Acid hydrolysis decreased growth and surfactant production except 0.5 wt% acid, which increased the yield by 25% over untreated effluent.

引用

页码：487 / 501

页数：15

共 23 条

[1]

ABDUL AS, 1990, GROUND WATER, V28, P290

[2] AQUEOUS SURFACTANT WASHING OF RESIDUAL OIL CONTAMINATION FROM SANDY SOIL [J].

ANG, CC ;

ABDUL, AS .

GROUND WATER MONITORING AND REMEDIATION, 1991, 11 (02) :121-127

[3]

[Anonymous], METHODS GEN MOL BACT

[4] SURFACTIN A CRYSTALLINE PEPTIDELIPID SURFACTANT PRODUCED BY BACILLUS SUBTILIS - ISOLATION CHARACTERIZATION AND ITS INHIBITION OF FIBRIN CLOT FORMATION [J].

ARIMA, K ;

KAKINUMA, A ;

TAMURA, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 31 (03) :488-&

[5]

ATLAS RM, 1993, HDB MICROBIOLOGICAL, P672

[6]

BAILEY JE, 1986, BIOCH ENG FUNDAMENTA, P5

[7] BIOSYNTHESIS OF ITURIN AND SURFACTIN BY BACILLUS-SUBTILIS - EVIDENCE FOR AMINO-ACID ACTIVATING ENZYMES [J].

BESSON, F ;

MICHEL, G .

BIOTECHNOLOGY LETTERS, 1992, 14 (11) :1013-1018

[8] ENHANCED PRODUCTION OF SURFACTIN FROM BACILLUS-SUBTILIS BY CONTINUOUS PRODUCT REMOVAL AND METAL CATION ADDITIONS [J].

COOPER, DG ;

MACDONALD, CR ;

DUFF, SJB ;

KOSARIC, N .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1981, 42 (03) :408-412

[9]

ELLIS WD, 1985, EPA600S285129 OFF RE

[10]

GEORGIOU G, 1992, BIO-TECHNOL, V10, P60, DOI 10.1038/nbt0192-60

← 1 2 3 →