""Turn-On" Sensing Behaviour of an In Situ Generated Fluorescein-Based Probe and Its Preferential Selectivity of Sodium Hypochlorite over tert-Butyl Hydroperoxide in Lung Adenocarcinoma Cells"

被引：11

作者：

Sandhu, Navjot ^{[1
]}

Saproo, Sheetanshu ^{[2
]}

Naidu, Srivatsava ^{[2
]}

Singh, Atul P. ^{[1
]}

Kumar, Kamlesh ^{[3
]}

Singh, Ashish P. ^{[4
]}

Yadav, Rajesh K. ^{[5
]}

机构：

[1] Chandigarh Univ, Dept Chem, Mohali, Punjab, India

[2] Indian Inst Technol, Ctr Biomed Engn CBME, Ropar, India

[3] CSIR Cent Sci Instruments Org, Ubiquitous Analyt Tech, Chandigarh, India

[4] BARC, Div Mat Grp, Mumbai, Maharashtra, India

[5] Madan Mohan Malaviya, Dept Appl Sci Chem, Gorakhpur, Uttar Pradesh, India

来源：

CHEMISTRYSELECT | 2020年 / 5卷 / 04期

关键词：

Adenocarcinoma cells; Fluorescent probe; Fluorescein; Oxidizing behaviour; t-BuOOH; Turn-on and Turn-off; TAP WATER; LUMINESCENT PROBES; HYDROGEN-PEROXIDE; CHEMODOSIMETER; OXIDATION; ACID; HOMOCYSTEINE; CHEMISTRY; HYDRAZIDE; CYSTEINE;

D O I：

10.1002/slct.201903843

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reactive Oxygen species (ROS) are well known for their oxidizing behaviour but it differs in fluorescein derivatives chemistry. With t-BuOOH, the probe F shows its unique turn-off sensing (A), while with NaOCl probe shows turn-on sensing property (B). In this study, it was observed that in situ generated species formed by the reaction of probe (F) with t-BuOOH is a unique molecule, having high turn-on behaviour in presence of sodium hypochlorite (approximate to 500 times). Further, fluorescence microscopy imaging using adenocarcinoma cells, A549 cells revealed that in-situ generated molecule, could be used as an efficient fluorescent probe for detecting NaOCl in living cells.

引用

页码：1264 / 1268

页数：5