Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection

被引：671
作者：
Chandran, K
Sullivan, NJ
Felbor, U
Whelan, SP
Cunningham, JM
机构：
[1] Brigham & Womens Hosp, Dept Med, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Boston, MA 02115 USA
[3] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA
[4] Biozentrum, Inst Human Genet, D-97074 Wurzburg, Germany
[5] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
来源：
SCIENCE | 2005年 / 308卷 / 5728期
关键词：
D O I：
10.1126/science.1110656
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Ebola virus (EboV) causes rapidly fatal hemorrhagic fever in humans and there is currently no effective treatment. We found that the infection of African green monkey kidney (Vero) cells by vesicular stornatitis viruses bearing the EboV gtycoprotein (GP) requires the activity of endosomal cysteine proteases. Using selective protease inhibitors and protease-deficient cell lines, we identified an essential role for cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry. Biochemical studies demonstrate that CatB and CatL mediate entry by carrying out proteolysis of the EboV GP subunit GP1 and support a multistep mechanism that explains the relative contributions of these enzymes to infection. CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs.
引用
收藏
页码：1643 / 1645
页数：3
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