Effects of Enhanced UV-B Radiation on the Cell Mitosis of the Callus in Wheat

被引:0
|
作者
Zhang, Mei-Ping [1 ]
Han, Rong [1 ]
Shan, Yong-Jie [1 ]
Li, Ya-ou [2 ]
Wu, Juan [2 ]
Tian, Wen-Hua [2 ]
Gao, Li-Mei [2 ]
机构
[1] Shanxi Normal Univ, Coll life Sci, Lin Fen 041004, Shan Xi, Peoples R China
[2] Shanxi Normal Univ, Coll urban & environm sci, Lin Fen 041004, Shan Xi, Peoples R China
来源
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年
基金
中国国家自然科学基金;
关键词
wheat; UV-B; chromosomal mutation;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In order to study effects of enhanced Ultraviolet-B (280nm-320nm) radiation on the cell mitosis, mature embryos of wheat as explant were cultured in the Murashige and Skoog (MS) medium with different concentration of 2, 4-D and 6-BA. The results indicated the inducing rate of the mature embryos from wheat on the MS with 2, 4-D 1.0 mg/L was higher and the callus was better than on the other Culture medium. The chromosomal mutation and mitosis of the callus in wheat were studied under the condition of the enhanced UV-B radiation. The results showed that enhanced UV-B radiation inhibited the cell division of callus in wheat, several types of chromosomal aberration such as micronucleus, multi-nucleus, nuclear aberration occurred during the interphase, which may be the results of UV-B inhabiting the DNA replication of the cell to lead to the cell DNA mutation and form the pyrimidine dimer.
引用
收藏
页码:1556 / +
页数:3
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