Simultaneous imaging of multiple focal planes using a two-photon scanning microscope

被引：73

作者：

Amir, W. ^{[1
]}

Carriles, R. ^{[1
]}

Hoover, E. E. ^{[1
]}

Planchon, T. A. ^{[1
]}

Durfee, C. G. ^{[1
]}

Squier, J. A. ^{[1
]}

机构：

[1] Colorado Sch Mines, Dept Phys, Golden, CO 80401 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 12期

关键词：

D O I：

10.1364/OL.32.001731

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Despite all the advances in nonlinear microscopy, all existing instruments are constrained to obtain images of one focal plane at a time. In this Letter we demonstrate a two-photon absorption fluorescence scanning microscope capable of imaging two focal planes simultaneously. This is accomplished by temporally demultiplexing the signal coming from two focal volumes at different sample depths. The scheme can be extended to three or more focal planes. (C) 2007 Optical Society of America

引用

页码：1731 / 1733

页数：3

共 8 条

[1] Simultaneous visualization of spatial and chromatic aberrations by two-dimensional Fourier transform spectral interferometry [J].