Sequence-specific covalent labelling of DNA

被引：22

作者：

Gottfried, Anna ^{[1
]}

Weinhold, Elmar ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Inst Organ Chem, D-52056 Aachen, Germany

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2011年 / 39卷

关键词：

DNA methyltransferase (DNA MTase); hairpin polyamide; nicking endonuclease (NEase); S-adenosylmethionine analogue (AdoMet analogue); triple-helix-forming oligonucleolide; INTERSTRAND CROSS-LINKING; SINGLE-MOLECULE; MINOR-GROOVE; PLASMID DNA; PADLOCK OLIGONUCLEOTIDES; FLUORESCENCE DETECTION; SUPERHELICAL DNA; RECOGNITION; SITE; TOOL;

D O I：

10.1042/BST0390623

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence-specific DNA modification is of significance for applications in bio- and nano-technology, medical diagnostics and fundamental life sciences research. Preferentially, labelling should be performed covalently, which avoids doubts about label dissociation from the DNA under various conditions. Several methods to label native DNA have been developed in the last two decades. Triple-helix-forming oligodeoxynucleotides and hairpin polyamides that bind DNA sequences specifically in the major and minor groove respectively were used as targeting devices for subsequent covalent labelling. In addition, enzyme-directed labelling approaches utilizing nicking endonucleases in combination with DNA polymerases or DNA methyltransferases have been employed. This review summarizes various techniques useful for functionalization of long native DNA.

引用

页码：623 / 628

页数：6