Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry

被引：16

作者：

Hwang, Heeyoun ^{[1
,2
,3
]}

Jeong, Ji Eun ^{[1
,2
]}

Lee, Hyun Kyoung ^{[1
,2
]}

Yun, Ki Na ^{[1
,4
]}

An, Hyun Joo ^{[2
,3
]}

Lee, Bonghee ^{[5
,6
,7
]}

Paik, Young-Ki ^{[8
,9
,10
]}

Jeong, Tae Seok ^{[11
]}

Yee, Gi Taek ^{[11
]}

Kim, Jin Young ^{[1
]}

Yoo, Jong Shin ^{[1
,2
]}

机构：

[1] Korea Basic Sci Inst, Biomed Omics Res, Cheongju, South Korea

[2] Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Daejeon, South Korea

[3] Chungnam Natl Univ, Asia Pacific Glyc Reference Site, Daejeon, South Korea

[4] Sogang Univ, Dept Chem, Seoul, South Korea

[5] Gachon Univ, Grad Sch Med, Dept Anat & Cell Biol, Incheon, South Korea

[6] Gachon Univ, Ctr Genom & Prote, Incheon, South Korea

[7] Gachon Univ, Lee Gil Ya Canc & Diabet Inst, Stem Cell Core Facil, Incheon, South Korea

[8] Yonsei Univ, Yonsei Proteome Res Ctr, Coll Life Sci & Biotechnol, Seoul, South Korea

[9] Yonsei Univ, Dept Integrated OMICS Biomed Sci, Coll Life Sci & Biotechnol, Seoul, South Korea

[10] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul, South Korea

[11] Gachon Univ, Dept Neurosurg, Gil Med Ctr, Incheon, South Korea

来源：

JOURNAL OF PROTEOME RESEARCH | 2018年 / 17卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1021/acs.jproteome.8b00408

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (POC7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).

引用

页码：4320 / 4324

页数：5

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