Confocal microscopy in transmitted light

被引：1

作者：

Dodt, HU ^{[1
]}

Becker, K ^{[1
]}

机构：

[1] Max Planck Inst Psychiat, Munich, Germany

来源：

CONFOCAL, MULTIPHOTON, AND NONLINEAR MICROSCOPIC IMAGING | 2003年 / 5139卷

关键词：

confocal microscopy; transmitted light; Nipkow disk; electronic pinhole; phase object;

D O I：

10.1117/12.499676

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We developed a confocal microscope for transmitted light to visualize fine details in phase objects like unstained biological specimens. The main difficulty of confocal microscopy in transmitted light is the alignment of illumination and detector pinholes. This alignment was achieved by using "electronic pinholes" on the detector side. In a first step, we were able to image cells in onion skin at greater depths, and with higher resolution, than by using conventional microscopy.

引用

页码：79 / 87

页数：9