Determination of oligonucleotide molecular masses by MALDI mass spectrometry

被引：2
作者：
Streletskii, AV
Kozlova, AY
Esipov, DS
Kayushin, AL
Korosteleva, MD
Esipov, SE
机构：
[1] Moscow MV Lomonosov State Univ, Fac Biol, Chair Bioorgan Chem, Moscow 119992, Russia
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2005年 / 31卷 / 02期
关键词：
MALDI mass spectrometry; modified oligonucleotides; oligonucleotides;
D O I：
10.1007/s11171-005-0019-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MALDI mass spectrometry (MS) of 14- to 42-mer homogeneous oligonucleotides and their mixtures was carried out using a Vision 2000 instrument (Thermo BioAnalysis, Finnigan, United States). Conditions for the determination of oligonucleotide molecular masses were optimized by applying various matrices and operation modes. The most reproducible results with minimal uncontrolled decomposition of the oligonucleotides including their apurinization during the MALDI MS registration were obtained using 2,4,6-trihydroxyacetophenone as a matrix instead of 3-hydroxypicolinic acid usually employed in the mass spectrometry of oligonucleotides. Our approach allows the determination of molecular masses of oligonucleotides obtained by chemical synthesis and the evaluation of their component composition and purity. It was applied to the mass spectrometric analysis of oligonucleotides containing a 3'-(methyl-C-phosphonate) group or a modified 1,N-6-ethenodeoxyadenosine unit.
引用
收藏
页码：139 / 145
页数：7
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