Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine

被引：0

作者：

King, Simon ^{[1
]}

Flannery, John ^{[1
]}

Batten, Carrie ^{[1
]}

Rajko-Nenow, Paulina ^{[1
]}

机构：

[1] Pirbright Inst, Ash Rd, Pirbright GU24 0NF, Surrey, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2021年 / 298卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Bluetongue virus; RT-qPCR; Serotyping; Diagnostics; PCR ASSAY; SEROTYPE; DISEASE; GOATS; CONTAMINATION;

D O I：

10.1016/j.jviromet.2021.114288

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), possibly representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (targeting genome segment 2) for these two new BTV strains. The limit of detection of both assays was 10 genome copies/mu l and no cross-reactivity with other BTV genotypes was observed. The performance of three other BTV group-specific diagnostic assays was also tested against the putative novel genotypes. RT-qPCR assays targeting BTV segment 9 and 10 detected both strains (SPvvvv/02 and SPvvvv/03) whereas a BTV segment 1 RTqPCR assay was unable to detect either BTV strain. The work presented here expands upon the current repertoire of RT-qPCR assays for BTV genotype determination.

引用

页数：6

共 32 条

[1] BLUETONGUE DISEASE IN DOGS ASSOCIATED WITH CONTAMINATED VACCINE [J].

AKITA, GY ;

IANCONESCU, M ;

MACLACHLAN, NJ ;

OSBURN, BI ;

GREENE, RT .

VETERINARY RECORD, 1994, 134 (11) :283-284

[2] Viral contamination in biologic manufacture and implications for emerging therapies [J].

Barone, Paul W. ;

Wiebe, Michael E. ;

Leung, James C. ;

Hussein, Islam T. M. ;

Keumurian, Flora J. ;

Bouressa, James ;

Brussel, Audrey ;

Chen, Dayue ;

Chong, Ming ;

Dehghani, Houman ;

Gerentes, Lionel ;

Gilbert, James ;

Gold, Dan ;

Kiss, Robert ;

Kreil, Thomas R. ;

Labatut, Rene ;

Li, Yuling ;

Muellberg, Juergen ;

Mallet, Laurent ;

Menzel, Christian ;

Moody, Mark ;

Monpoeho, Serge ;

Murphy, Marie ;

Plavsic, Mark ;

Roth, Nathan J. ;

Roush, David ;

Ruffing, Michael ;

Schicho, Richard ;

Snyder, Richard ;

Stark, Daniel ;

Zhang, Chun ;

Wolfrum, Jacqueline ;

Sinskey, Anthony J. ;

Springs, Stacy L. .

NATURE BIOTECHNOLOGY, 2020, 38 (05) :563-572

[3] Evidence for Transmission of Bluetongue Virus Serotype 26 through Direct Contact [J].

Batten, Carrie ;

Darpel, Karin ;

Henstock, Mark ;

Fay, Petra ;

Veronesi, Eva ;

Gubbins, Simon ;

Graves, Samantha ;

Frost, Lorraine ;

Oura, Christopher .

PLOS ONE, 2014, 9 (05)

[4] Bluetongue virus serotype 27: Experimental infection of goats, sheep and cattle with three BTV-27 variants reveal atypical characteristics and likely direct contact transmission BTV-27 between goats [J].

Breard, E. ;

Schulz, C. ;

Sailleau, C. ;

Bernelin-Cottet, C. ;

Viarouge, C. ;

Vitour, D. ;

Guillaume, B. ;

Caignard, G. ;

Gorlier, A. ;

Attoui, H. ;

Gallois, M. ;

Hoffmann, B. ;

Zientara, S. ;

Beer, M. .

TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 (02) :e251-e263

[5] Characterization of bluetongue virus serotype 28 [J].

Bumbarov, Velizar ;

Golender, Natalia ;

Jenckel, Maria ;

Wernike, Kerstin ;

Beer, Martin ;

Khinich, Evgeny ;

Zalesky, Olga ;

Erster, Oran .

TRANSBOUNDARY AND EMERGING DISEASES, 2020, 67 (01) :171-182

[6] Detection and isolation of Bluetongue virus from commercial vaccine batches [J].

Bumbarov, Velizar ;

Golender, Natalia ;

Erster, Oran ;

Khinich, Yevgeny .

VACCINE, 2016, 34 (28) :3317-3323

[7]

Burstyn DG, 1996, DEV BIOL STAND, V88, P199

[8] Toggenburg Orbivirus, a new bluetongue virus: Initial detection, first observations in field and experimental infection of goats and sheep [J].

Chaignat, Valerie ;

Worwa, Gabriella ;

Scherrer, Nicole ;

Hilbe, Monika ;

Ehrensperger, Felix ;

Batten, Carrie ;

Cortyen, Mandy ;

Hofmann, Martin ;

Thuer, Barbara .

VETERINARY MICROBIOLOGY, 2009, 138 (1-2) :11-19

[9] Improved PCR diagnostics using up-to-date in silico validation: An F-gene RT-qPCR assay for the detection of all four lineages of peste des petits ruminants virus [J].

Flannery, John ;

Rajko-Nenow, Paulina ;

Arnold, Hannah ;

van Weezep, Erik ;

van Rijn, Piet A. ;

Ngeleja, Chanasa ;

Batten, Carrie .

JOURNAL OF VIROLOGICAL METHODS, 2019, 274

[10] Economic Impact of a Bluetongue Serotype 8 Epidemic in Germany [J].

Gethmann, Joern ;

Probst, Carolina ;

Conraths, Franz J. .

FRONTIERS IN VETERINARY SCIENCE, 2020, 7

← 1 2 3 4 →