Protection against fowl cholera in ducks immunized with a combination vaccine containing live attenuated duck enteritis virus and recombinant outer membrane protein H of Pasteurella multocida

被引:14
作者
Apinda, Nisachon [1 ]
Nambooppha, Boondarika [1 ]
Rittipornlertrak, Amarin [1 ]
Tankaew, Pallop [2 ]
Punyapornwithaya, Veerasak [3 ]
Nair, Venugopal [4 ,5 ]
Sawada, Takuo [6 ]
Sthitmatee, Nattawooti [1 ,7 ]
机构
[1] Chiang Mai Univ, Dept Vet Biosci & Publ Hlth, Fac Vet Med, Chiang Mai, Thailand
[2] Chiang Mai Univ, Cent Lab, Fac Vet Med, Chiang Mai, Thailand
[3] Chiang Mai Univ, Fac Vet Med, Dept Food Anim Clin, Chiang Mai, Thailand
[4] Pirbright Inst, Pirbright, Surrey, England
[5] Univ Oxford, Dept Zool, Oxford, England
[6] Nippon Vet & Life Sci Univ, Lab Vet Microbiol, Musashino, Tokyo, Japan
[7] Chiang Mai Univ, Excellence Ctr Vet Biosci, Chiang Mai, Thailand
关键词
Combination vaccine; duck enteritis virus; duck plague; fowl cholera; Pasteurella multocida; protection; CAPSULAR PROTEIN; INDIRECT ELISA; PLAGUE; CHICKENS; STRAIN;
D O I
10.1080/03079457.2019.1711020
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Fowl cholera is a highly contagious disease within the global duck farming industry. This study aimed at formulating and evaluating the protective efficacy of a combination vaccine containing a recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 with a live attenuated duck plague vaccine into a single dose. Four groups of ducks received different treatments and the groups were labelled as non-vaccinated, combined vaccination, duck plague vaccination and rOmpH vaccination, respectively. The combined vaccination group was comprised of live attenuated duck plague commercial vaccine with 100 mu g rOmpH to a total volume of 0.5 ml/duck/intramuscular administration. All groups were challenged with avian P. multocida strain X-73 via intranasal administration. In addition, blood samples were collected monthly over a period of 6 months to determine the appropriate antibody level by indirect ELISA. The indirect ELISA results in the combination vaccine group revealed that the average levels of the serum antibody against the duck enteritis virus (0.477 +/- 0.155) and fowl cholera (0.383 +/- 0.100) were significantly higher than those values in the non-vaccinated control group (0.080 +/- 0.027 and 0.052 +/- 0.017), respectively (P < 0.05). Moreover, all vaccinated ducks were effectively protected from fowl cholera. This preliminary study indicated that a combination vaccine did not affect the antibody response in the subjects while protecting the ducks against experimental P. multocida infection. This combination vaccine should be considered part of an alternative pre-treatment strategy that could replace the monovalent vaccine.
引用
收藏
页码:221 / 229
页数:9
相关论文
共 39 条
[1]  
[Anonymous], 2013, R Package Version
[2]  
Avian Influenza, 2015, OIE TERR MAN
[3]   Immune responses against enterovirus A71 infection: Implications for vaccine success [J].
Aw-Yong, Kam Leng ;
NikNadia, Nik Mohd Nasir ;
Tan, Chee Wah ;
Sam, I-Ching ;
Chan, Yoke Fun .
REVIEWS IN MEDICAL VIROLOGY, 2019, 29 (05)
[4]  
Belloc C, 2008, REV MED VET-TOULOUSE, V159, P371
[5]  
Borrathybay E., 2008, Bulletin of Nippon Veterinary and Life Science University, P90
[6]   PURIFICATION AND CHARACTERIZATION OF PROTEIN-H, THE MAJOR PORIN OF PASTEURELLA-MULTOCIDA [J].
CHEVALIER, G ;
DUCHLOHIER, H ;
THOMAS, D ;
SHECHTER, E ;
WROBLEWSKI, H .
JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :266-276
[7]   Duck plague epizootics in the United States, 1967-1995 [J].
Converse, KA ;
Kidd, GA .
JOURNAL OF WILDLIFE DISEASES, 2001, 37 (02) :347-357
[8]  
Crowther J.R., 2001, The ELISA guidebook
[9]   Duck virus enteritis (duck plague) - a comprehensive update [J].
Dhama, Kuldeep ;
Kumar, Naveen ;
Saminathan, Mani ;
Tiwari, Ruchi ;
Karthik, Kumaragurubaran ;
Kumar, M. Asok ;
Palanivelu, M. ;
Shabbir, Muhammad Zubair ;
Malik, Yashpal Singh ;
Singh, Raj Kumar .
VETERINARY QUARTERLY, 2017, 37 (01) :57-80
[10]  
Hanan A., 2014, NAT SCI, V12, P142